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Anti-RUNX3 Picoband Antibody

     
  • WB - Anti-RUNX3 Picoband Antibody ABO12122
    Anti- RUNX3 Picoband antibody, ABO12122, Western blottingAll lanes: Anti RUNX3 (ABO12122) at 0.5ug/mlLane 1: A431 Whole Cell Lysate at 40ugLane 2: U20S Whole Cell Lysate at 40ugPredicted bind size: 44KDObserved bind size: 44KD
  • IHC - Anti-RUNX3 Picoband Antibody ABO12122
    Anti- RUNX3 Picoband antibody, ABO12122, IHC(P)IHC(P): Mouse Spleen Tissue
  • IHC - Anti-RUNX3 Picoband Antibody ABO12122
    Anti- RUNX3 Picoband antibody, ABO12122, IHC(P)IHC(P): Rat Spleen Tissue
  • IHC - Anti-RUNX3 Picoband Antibody ABO12122
    Anti- RUNX3 Picoband antibody, ABO12122, IHC(P)IHC(P): Human Intestinal Cancer Tissue
  • IHC - Anti-RUNX3 Picoband Antibody ABO12122
    Anti- RUNX3 Picoband antibody, ABO12122, IHC(P)IHC(P): Human Lung Cancer Tissue
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P
Primary Accession Q13761
Host Rabbit
Reactivity Human, Mouse, Rat
Clonality Polyclonal
Format Lyophilized
Description Rabbit IgG polyclonal antibody for Runt-related transcription factor 3(RUNX3) detection. Tested with WB, IHC-P in Human;Mouse;Rat.
Reconstitution Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Additional Information
Gene ID 864
Other Names Runt-related transcription factor 3, Acute myeloid leukemia 2 protein, Core-binding factor subunit alpha-3, CBF-alpha-3, Oncogene AML-2, Polyomavirus enhancer-binding protein 2 alpha C subunit, PEA2-alpha C, PEBP2-alpha C, SL3-3 enhancer factor 1 alpha C subunit, SL3/AKV core-binding factor alpha C subunit, RUNX3, AML2, CBFA3, PEBP2A3
Calculated MW 44356 MW KDa
Application Details Immunohistochemistry(Paraffin-embedded Section), 0.5-1 µg/ml, Human, Mouse, Rat, By Heat
Western blot, 0.1-0.5 µg/ml, Human
Subcellular Localization Nucleus . Cytoplasm . The tyrosine phosphorylated form localizes to the cytoplasm.
Protein Name Runt-related transcription factor 3
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Immunogen E.coli-derived human RUNX3 recombinant protein (Position: M128-Y270). Human RUNX3 shares 93% amino acid (aa) sequence identity with mouse RUNX3.
Purification Immunogen affinity purified.
Cross Reactivity No cross reactivity with other proteins
Storage At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Protein Information
Name RUNX3
Synonyms AML2, CBFA3, PEBP2A3
Function Forms the heterodimeric complex core-binding factor (CBF) with CBFB. RUNX members modulate the transcription of their target genes through recognizing the core consensus binding sequence 5'-TGTGGT-3', or very rarely, 5'-TGCGGT-3', within their regulatory regions via their runt domain, while CBFB is a non-DNA- binding regulatory subunit that allosterically enhances the sequence-specific DNA-binding capacity of RUNX. The heterodimers bind to the core site of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL3 and GM-CSF promoters (By similarity). May be involved in the control of cellular proliferation and/or differentiation. In association with ZFHX3, upregulates CDKN1A promoter activity following TGF-beta stimulation (PubMed:20599712). CBF complexes repress ZBTB7B transcription factor during cytotoxic (CD8+) T cell development. They bind to RUNX-binding sequence within the ZBTB7B locus acting as transcriptional silencer and allowing for cytotoxic T cell differentiation. CBF complexes binding to the transcriptional silencer is essential for recruitment of nuclear protein complexes that catalyze epigenetic modifications to establish epigenetic ZBTB7B silencing (By similarity).
Cellular Location Nucleus {ECO:0000255|PROSITE- ProRule:PRU00399, ECO:0000269|PubMed:20100835, ECO:0000269|PubMed:20599712}. Cytoplasm Note=The tyrosine phosphorylated form localizes to the cytoplasm Translocates from the cytoplasm to the nucleus following TGF-beta stimulation.
Tissue Location Expressed in gastric cancer tissues (at protein level).
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Background

Runt-related transcription factor 3, also called AML2 is a protein that in humans is encoded by the RUNX3 gene. The RUNX3 gene encodes a Runt-related transcription factor, which is part of the RUNX gene family. By fluorescence in situ hybridization, RUNX3 was assigned to human chromosome 1p36.11. RUNX3 binds to the core site of murine Leukemia virus, the core sequences in the enhancer of the polyomavirus, and also to the enhancers of the T-cell receptor genes. It may be involved in the control of cellular proliferation and/or differentiation.

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$ 280.00
Cat# ABO12122
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