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Anti-SMAD6 Picoband Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB |
|---|---|
| Primary Accession | O43541 |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Clonality | Polyclonal |
| Format | Lyophilized |
| Description | Rabbit IgG polyclonal antibody for Mothers against decapentaplegic homolog 6(SMAD6) detection. Tested with WB in Human;Mouse;Rat. |
| Reconstitution | Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
| Gene ID | 4091 |
|---|---|
| Other Names | Mothers against decapentaplegic homolog 6, MAD homolog 6, Mothers against DPP homolog 6, SMAD family member 6, SMAD 6, Smad6, hSMAD6, SMAD6, MADH6 |
| Calculated MW | 53497 MW KDa |
| Application Details | Western blot, 0.1-0.5 µg/ml, Human, Mouse, Rat |
| Subcellular Localization | Nucleus . |
| Tissue Specificity | Ubiquitous in various organs, with higher levels in lung. Isoform B is up-regulated in diseased heart tissue. |
| Protein Name | Mothers against decapentaplegic homolog 6 |
| Contents | Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3. |
| Immunogen | E.coli-derived human SMAD6 recombinant protein (Position: E131-D456). Human SMAD6 shares 95.3% amino acid (aa) sequence identity with mouse SMAD6. |
| Purification | Immunogen affinity purified. |
| Cross Reactivity | No cross reactivity with other proteins |
| Storage | At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing. |
| Sequence Similarities | Belongs to the dwarfin/SMAD family. |
| Name | SMAD6 |
|---|---|
| Synonyms | MADH6 |
| Function | Transforming growth factor-beta superfamily receptors signaling occurs through the Smad family of intracellular mediators. SMAD6 is an inhibitory Smad (i-Smad) that negatively regulates signaling downstream of type I transforming growth factor-beta (PubMed:9436979, PubMed:16951688, PubMed:22275001, PubMed:9759503, PubMed:10647776, PubMed:10708948, PubMed:10708949, PubMed:30848080). Acts as a mediator of TGF-beta and BMP anti-inflammatory activities. Suppresses IL1R-TLR signaling through its direct interaction with PEL1, preventing NF-kappa-B activation, nuclear transport and NF-kappa-B- mediated expression of pro-inflammatory genes (PubMed:16951688). Blocks the BMP-SMAD1 signaling pathway by competing with SMAD4 for receptor- activated SMAD1-binding (PubMed:9436979, PubMed:30848080). Binds to regulatory elements in target promoter regions (PubMed:16491121). |
| Cellular Location | Nucleus. |
| Tissue Location | [Isoform B]: Expressed in the brain, heart, ovary, peripheral blood leukocytes, small intestine, spleen, thymus, bone marrow, fetal liver and lymph nodes. |
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Provided below are standard protocols that you may find useful for product applications.
Background
Mothers against decapentaplegic homolog 6, also known as SMAD6, is a protein that in humans is encoded by the SMAD6 gene. It belongs to the SMAD family of proteins, which belongs to the TGFβ superfamily of modulators. By PCR screening of a somatic cell hybrid panel and YACs, this gene was mapped to 15q22.31. And this gene acts as a mediator of TGF-beta and BMP antiflammatory activity. It can suppress 1L 1R-TLR signaling through its direct interaction with PEL1 and prevent NF-kappa-B activation, nuclear transport and NF-kappa-B-mediated expression of proinflammatory genes. SMAD6 may play a role in blocking the BMP-SMAD1 signaling pathway by competing with SMAD4 for receptor-activated SMAD1-binding and binding to regulatory elements in target promoter regions.
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