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Anti-MICB Picoband Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB |
|---|---|
| Primary Accession | Q29980 |
| Host | Rabbit |
| Reactivity | Human, Rat |
| Clonality | Polyclonal |
| Format | Lyophilized |
| Description | Rabbit IgG polyclonal antibody for MHC class I polypeptide-related sequence B(MICB) detection. Tested with WB in Human;Rat. |
| Reconstitution | Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
| Gene ID | 4277 |
|---|---|
| Other Names | MHC class I polypeptide-related sequence B, MIC-B, MICB {ECO:0000312|EMBL:CAA62823.1} |
| Calculated MW | 42646 MW KDa |
| Application Details | Western blot, 0.1-0.5 µg/ml, Human, Rat |
| Subcellular Localization | Cell membrane ; Single-pass type I membrane protein . Binding to human cytomegalovirus glycoprotein UL16 causes sequestration in the endoplasmic reticulum. . |
| Tissue Specificity | Widely expressed with the exception of the central nervous system where it is absent. Expressed in many, but not all, epithelial tumors of lung, breast, kidney, ovary, prostate and colon. In hepatocellular carcinomas, expressed in tumor cells but not in surrounding non-cancerous tissue. . |
| Protein Name | MHC class I polypeptide-related sequence B |
| Contents | Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3. |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human MICB (23-48aa AEPHSLRYNLMVLSQDESVQSGFLAE). |
| Purification | Immunogen affinity purified. |
| Cross Reactivity | No cross reactivity with other proteins |
| Storage | At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing. |
| Sequence Similarities | Belongs to the MHC class I family. MIC subfamily. |
| Name | MICB {ECO:0000312|EMBL:CAA62823.1} |
|---|---|
| Function | Seems to have no role in antigen presentation. Acts as a stress-induced self-antigen that is recognized by gamma delta T cells. Ligand for the KLRK1/NKG2D receptor. Binding to KLRK1 leads to cell lysis. |
| Cellular Location | Cell membrane {ECO:0000250|UniProtKB:Q29983}; Single-pass type I membrane protein {ECO:0000250|UniProtKB:Q29983} Note=Binding to human cytomegalovirus glycoprotein UL16 causes sequestration in the endoplasmic reticulum {ECO:0000250|UniProtKB:Q29983, ECO:0000269|PubMed:12782710} |
| Tissue Location | Widely expressed with the exception of the central nervous system where it is absent. Expressed in many, but not all, epithelial tumors of lung, breast, kidney, ovary, prostate and colon In hepatocellular carcinomas, expressed in tumor cells but not in surrounding non-cancerous tissue. |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
MHC class I polypeptide-related sequence B is a protein that in humans is encoded by the MICB gene. This gene encodes a heavily glycosylated protein which is a ligand for the NKG2D type II receptor. Binding of the ligand activates the cytolytic response of natural killer (NK) cells, CD8 alphabeta T cells, and gammadelta T cells which express the receptor. This protein is stress-induced and is similar to MHC class I molecules; however, it does not associate with beta-2-microglobulin or bind peptides. Alternative splicing results in multiple transcript variants.
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