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Anti-APOA1 Antibody

     
  • WB - Anti-APOA1 Antibody ABO12531
    Western blot analysis of APOA1 expression in rat liver extract (lane 1) and rat testis extract (lane 2). APOA1 at 24KD was detected using rabbit anti- APOA1 Antigen Affinity purified polyclonal antibody(Catalog # ABO12531) at 0.5 ??g/mL. The blot was developed using chemiluminescence (ECL) method .
  • IHC - Anti-APOA1 Antibody ABO12531
    APOA1 was detected in paraffin-embedded sections of rat liver tissues using rabbit anti- APOA1 Antigen Affinity purified polyclonal antibody (Catalog # ABO12531) at 1 μg/mL. The immunohistochemical section was developed using SABC method .
  • IHC - Anti-APOA1 Antibody ABO12531
    APOA1 was detected in paraffin-embedded sections of rat kidney tissues using rabbit anti- APOA1 Antigen Affinity purified polyclonal antibody (Catalog # ABO12531) at 1 μg/mL. The immunohistochemical section was developed using SABC method .
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P
Primary Accession P04639
Host Rabbit
Reactivity Rat
Clonality Polyclonal
Format Lyophilized
Description Rabbit IgG polyclonal antibody for Apolipoprotein A-I(APOA1) detection. Tested with WB, IHC-P in Rat.
Reconstitution Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Additional Information
Gene ID 25081
Other Names Apolipoprotein A-I, Apo-AI, ApoA-I, Apolipoprotein A1, Proapolipoprotein A-I, ProapoA-I, Apoa1
Calculated MW 30062 MW KDa
Application Details Immunohistochemistry(Paraffin-embedded Section), 0.5-1 µg/ml, Rat, By Heat

Western blot, 0.1-0.5 µg/ml, Rat
Subcellular Localization Secreted.
Tissue Specificity Major protein of plasma HDL, also found in chylomicrons.
Protein Name Apolipoprotein A-I
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Immunogen E. coli-derived rat APOA1 recombinant protein (Position: D25-A259). Rat APOA1 shares 61.7% and 68.6% amino acid (aa) sequence identity with human and mouse APOA1, respectively.
Purification Immunogen affinity purified.
Cross Reactivity No cross reactivity with other proteins.
Storage At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Protein Information
Name Apoa1
Function Participates in the reverse transport of cholesterol from tissues to the liver for excretion by promoting cholesterol efflux from tissues and by acting as a cofactor for the lecithin cholesterol acyltransferase (LCAT). As part of the SPAP complex, activates spermatozoa motility.
Cellular Location Secreted.
Tissue Location Major protein of plasma HDL, also found in chylomicrons
Citations (0)

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Background

Apolipoprotein A-1, also known as APOA1, is a human protein with a specific role in lipid metabolism. It binds to lipopolysaccharide or endotoxin, and has a major role in the anti-endotoxin function of HDL. The gene is mapped to 11q23. And it is a single polypeptide chain with 243 amino acid residues of known primary amino acid sequence. The ApoA-I protein promotes cholesterol efflux from tissues to the liver for excretion. It is a cofactor for lecithin cholesterolacyltransferase (LCAT) which is responsible for the formation of most plasma cholesteryl esters. ApoA-I is also isolated as a prostacyclin (PGI2) stabilizing factor, and thus may have an anticlotting effect. Defects in the gene encoding it are associated with HDL deficiencies, including Tangier disease, and with systemic non-neuropathic amyloidosis. Additionally, ApoA-I overexpression promotes macrophage-specific reverse cholesterol transport.

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$ 280.00
Cat# ABO12531
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