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Anti-EREG Picoband Antibody

     
  • WB - Anti-EREG Picoband Antibody ABO12550
    Western blot analysis of EREG expression in human placenta extract (lane 1) and HEPG2 whole cell lysates (lane 2). EREG at 20KD was detected using rabbit anti- EREG Antigen Affinity purified polyclonal antibody (Catalog # ABO12550) at0.5 ??g/mL. The blot was developed using chemiluminescence (ECL) method .
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB
Primary Accession O14944
Host Rabbit
Reactivity Human
Clonality Polyclonal
Format Lyophilized
Description Rabbit IgG polyclonal antibody for Proepiregulin(EREG) detection. Tested with WB in Human.
Reconstitution Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Additional Information
Other Names Proepiregulin, Epiregulin, EPR, EREG
Calculated MW 19044 MW KDa
Application Details Western blot, 0.1-0.5 µg/ml, Human
Subcellular Localization Epiregulin: Secreted, extracellular space .
Tissue Specificity In normal adults, expressed predominantly in the placenta and peripheral blood leukocytes. High levels were detected in carcinomas of the bladder, lung, kidney and colon. .
Protein Name Proepiregulin
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Immunogen E. coli-derived human EREG recombinant protein (Position: V60-L108). Human EREG shares 85.7% and 87.8% amino acid (aa) sequence identity with mouse and rat EREG, respectively.
Purification Immunogen affinity purified.
Cross Reactivity No cross reactivity with other proteins.
Storage At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Protein Information
Name EREG
Function Ligand of the EGF receptor/EGFR and ERBB4. Stimulates EGFR and ERBB4 tyrosine phosphorylation (PubMed:9419975). Contributes to inflammation, wound healing, tissue repair, and oocyte maturation by regulating angiogenesis and vascular remodeling and by stimulating cell proliferation (PubMed:24631357).
Cellular Location Epiregulin: Secreted, extracellular space
Tissue Location In normal adults, expressed predominantly in the placenta and peripheral blood leukocytes. High levels were detected in carcinomas of the bladder, lung, kidney and colon EMBL; D30783; BAA22146.1; -; mRNA EMBL; AK314959; BAG37463.1; -; mRNA EMBL; CR541887; CAG46685.1; -; mRNA EMBL; CH471057; EAX05709.1; -; Genomic_DNA EMBL; BC136404; AAI36405.1; -; mRNA EMBL; BC136405; AAI36406.1; -; mRNA CCDS; CCDS3564.1; - RefSeq; NP_001423.1; NM_001432.2 UniGene; Hs.115263; - PDB; 1K36; NMR; -; A=63-108 PDB; 1K37; NMR; -; A=63-108 PDB; 5E8D; X-ray; 2.50 A; A=38-108 PDB; 5WB7; X-ray; 2.94 A; E/F/G/H=56-116 PDBsum; 1K36; - PDBsum; 1K37; - PDBsum; 5E8D; - PDBsum; 5WB7; - ProteinModelPortal; O14944; - SMR; O14944; - BioGrid; 108381; 5 IntAct; O14944; 3 STRING; 9606.ENSP00000244869; - iPTMnet; O14944; - PhosphoSitePlus; O14944; - BioMuta; EREG; - MaxQB; O14944; - PaxDb; O14944; - PeptideAtlas; O14944; - PRIDE; O14944; - ProteomicsDB; 48327; - DNASU; 2069; - Ensembl; ENST00000244869; ENSP00000244869; ENSG00000124882 GeneID; 2069; - KEGG; hsa:2069; - UCSC; uc003hie.2; human CTD; 2069; - DisGeNET; 2069; - EuPathDB; HostDB:ENSG00000124882.3; - GeneCards; EREG; - HGNC; HGNC:3443; EREG HPA; HPA054373; - MIM; 602061; gene neXtProt; NX_O14944; - OpenTargets; ENSG00000124882; - PharmGKB; PA27856; - eggNOG; ENOG410IXV0; Eukaryota eggNOG; ENOG410YNFA; LUCA GeneTree; ENSGT00510000048748; - HOGENOM; HOG000059635; - HOVERGEN; HBG005601; - InParanoid; O14944; - KO; K09784; - OMA; RCEHFFL; - OrthoDB; EOG091G0SX3; - PhylomeDB; O14944; - TreeFam; TF336145; - Reactome; R-HSA-1227986; Signaling by ERBB2 Reactome; R-HSA-1236394; Signaling by ERBB4 Reactome; R-HSA-1250196; SHC1 events in ERBB2 signaling Reactome; R-HSA-1250342; PI3K events in ERBB4 signaling Reactome; R-HSA-1250347; SHC1 events in ERBB4 signaling Reactome; R-HSA-1251985; Nuclear signaling by ERBB4 Reactome; R-HSA-1257604; PIP3 activates AKT signaling Reactome; R-HSA-1963640; GRB2 events in ERBB2 signaling Reactome; R-HSA-1963642; PI3K events in ERBB2 signaling Reactome; R-HSA-2219530; Constitutive Signaling by Aberrant PI3K in Cancer Reactome; R-HSA-5673001; RAF/MAP kinase cascade Reactome; R-HSA-6785631; ERBB2 Regulates Cell Motility Reactome; R-HSA-6811558; PI5P, PP2A and IER3 Regulate PI3K/AKT Signaling Reactome; R-HSA-8847993; ERBB2 Activates PTK6 Signaling Reactome; R-HSA-8863795; Downregulation of ERBB2 signaling SIGNOR; O14944; - ChiTaRS; EREG; human EvolutionaryTrace; O14944; - GeneWiki; Epiregulin; - GenomeRNAi; 2069; - PRO; PR:O14944; - Proteomes; UP000005640; Chromosome 4 Bgee; ENSG00000124882; - CleanEx; HS_EREG; - Genevisible; O14944; HS GO; GO:0005576; C:extracellular region; TAS:Reactome GO; GO:0005615; C:extracellular space; IDA:UniProtKB GO; GO:0005887; C:integral component of plasma membrane; TAS:UniProtKB GO; GO:0005622; C:intracellular; IEA:GOC GO; GO:0005154; F:epidermal growth factor receptor binding; ISS:UniProtKB GO; GO:0008083; F:growth factor activity; IBA:GO_Central GO; GO:0046934; F:phosphatidylinositol-4,5-bisphosphate 3-kinase activity; TAS:Reactome GO; GO:0004713; F:protein tyrosine kinase activity; TAS:Reactome GO; GO:0005088; F:Ras guanyl-nucleotide exchange factor activity; TAS:Reactome GO; GO:0009653; P:anatomical structure morphogenesis; TAS:UniProtKB GO; GO:0001525; P:angiogenesis; IEA:UniProtKB-KW GO; GO:0009887; P:animal organ morphogenesis; TAS:UniProtKB GO; GO:0007267; P:cell-cell signaling; IDA:UniProtKB GO; GO:0019221; P:cytokine-mediated signaling pathway; IDA:UniProtKB GO; GO:0007173; P:epidermal growth factor receptor signaling pathway; ISS:UniProtKB GO; GO:0038128; P:ERBB2 signaling pathway; TAS:Reactome GO; GO:0007143; P:female meiotic nuclear division; ISS:UniProtKB GO; GO:0030216; P:keratinocyte differentiation; TAS:UniProtKB GO; GO:0043616; P:keratinocyte proliferation; IDA:UniProtKB GO; GO:0042700; P:luteinizing hormone signaling pathway; ISS:UniProtKB GO; GO:0000165; P:MAPK cascade; TAS:Reactome GO; GO:0009299; P:mRNA transcription; IDA:UniProtKB GO; GO:0008285; P:negative regulation of cell proliferation; ISS:UniProtKB GO; GO:0050680; P:negative regulation of epithelial cell proliferation; TAS:UniProtKB GO; GO:0051151; P:negative regulation of smooth muscle cell differentiation; IDA:UniProtKB GO; GO:0045892; P:negative regulation of transcription, DNA-templated; IDA:UniProtKB GO; GO:0001556; P:oocyte maturation; ISS:UniProtKB GO; GO:0001550; P:ovarian cumulus expansion; ISS:UniProtKB GO; GO:0030728; P:ovulation; ISS:UniProtKB GO; GO:0051781; P:positive regulation of cell division; IEA:UniProtKB-KW GO; GO:0008284; P:positive regulation of cell proliferation; IDA:UniProtKB GO; GO:0042108; P:positive regulation of cytokine biosynthetic process; IDA:UniProtKB GO; GO:0001819; P:positive regulation of cytokine production; ISS:UniProtKB GO; GO:0045740; P:positive regulation of DNA replication; IDA:UniProtKB GO; GO:0045741; P:positive regulation of epidermal growth factor-activated receptor activity; ISS:UniProtKB GO; GO:0048146; P:positive regulation of fibroblast proliferation; IDA:UniProtKB GO; GO:0045089; P:positive regulation of innate immune response; ISS:UniProtKB GO; GO:0045410; P:positive regulation of interleukin-6 biosynthetic process; ISS:UniProtKB GO; GO:0045840; P:positive regulation of mitotic nuclear division; ISS:UniProtKB GO; GO:0042327; P:positive regulation of phosphorylation; IDA:UniProtKB GO; GO:0045860; P:positive regulation of protein kinase activity; IDA:UniProtKB GO; GO:0051897; P:positive regulation of protein kinase B signaling; TAS:Reactome GO; GO:0048661; P:positive regulation of smooth muscle cell proliferation; ISS:UniProtKB GO; GO:0048160; P:primary follicle stage; ISS:UniProtKB GO; GO:2000145; P:regulation of cell motility; TAS:Reactome GO; GO:0043434; P:response to peptide hormone; IEA:Ensembl GO; GO:0042060; P:wound healing; TAS:UniProtKB InterPro; IPR013032; EGF-like_CS InterPro; IPR000742; EGF-like_dom InterPro; IPR015497; EGF_rcpt_ligand PANTHER; PTHR10740; PTHR10740; 1 PROSITE; PS00022; EGF_1; 1 PROSITE; PS01186; EGF_2; 1 PROSITE; PS50026; EGF_3; 1 1: Evidence at protein level; 3D-structure; Angiogenesis; Cell membrane; Complete proteome; Developmental protein; Differentiation; Disulfide bond; EGF-like domain; Glycoprotein; Growth factor; Membrane; Mitogen; Polymorphism; Reference proteome; Secreted; Signal; Transmembrane; Transmembrane helix SIGNAL 1 29 CHAIN 30 169 Proepiregulin /FTId=PRO_0000302800 PROPEP 30 62 {ECO:0000250|UniProtKB:Q61521, ECO:0000250|UniProtKB:Q9Z0L5} /FTId=PRO_0000007556 CHAIN 63 108 Epiregulin /FTId=PRO_0000007557 PROPEP 109 169 Removed in mature form. /FTId=PRO_0000007558 TOPO_DOM 60 119 Extracellular. TRANSMEM 120 140 Helical. TOPO_DOM 141 169 Cytoplasmic. DOMAIN 64 104 EGF-like. {ECO:0000255|PROSITE- ProRule:PRU00076} COMPBIAS 145 152 Arg/Lys-rich (basic) CARBOHYD 47 47 N-linked (GlcNAc...) asparagine DISULFID 68 81 {ECO:0000255|PROSITE-ProRule:PRU00076, ECO:0000269|PubMed:14572630} DISULFID 76 92 {ECO:0000255|PROSITE-ProRule:PRU00076, ECO:0000269|PubMed:14572630} DISULFID 94 103 {ECO:0000255|PROSITE-ProRule:PRU00076, ECO:0000269|PubMed:14572630} VARIANT 42 42 G -> A (in a breast cancer sample; somatic mutation) /FTId=VAR_035833 VARIANT 147 147 R -> Q (in dbSNP:rs35275884) /FTId=VAR_033827 STRAND 65 67 {ECO:0000244|PDB:5WB7} HELIX 70 72 {ECO:0000244|PDB:5E8D} TURN 73 75 {ECO:0000244|PDB:5E8D} STRAND 79 84 {ECO:0000244|PDB:5E8D} TURN 85 88 {ECO:0000244|PDB:5E8D} STRAND 89 94 {ECO:0000244|PDB:5E8D} STRAND 98 100 {ECO:0000244|PDB:5E8D} SEQUENCE 169 AA; 19044 MW; 17F3926ADFB2BDEE CRC64; MTAGRRMEML CAGRVPALLL CLGFHLLQAV LSTTVIPSCI PGESSDNCTA LVQTEDNPRV AQVSITKCSS DMNGYCLHGQ CIYLVDMSQN YCRCEVGYTG VRCEHFFLTV HQPLSKEYVA LTVILIILFL ITVVGSTYYF CRWYRNRKSK EPKKEYERVT SGDPELPQV
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Background

Epiregulin (EPR) is a protein that in humans is encoded by the EREG gene. It is mapped to 4q13.3. This gene encodes a secreted peptide hormone and member of the epidermal growth factor (EGF) family of proteins. The encoded protein is a ligand of the epidermal growth factor receptor (EGFR) and the structurally related erb-b2 receptor tyrosine kinase 4 (ERBB4). Also, it may be involved in a wide range of biological processes including inflammation, wound healing, oocyte maturation, and cell proliferation. Additionally, the encoded protein may promote the progression of cancers of various human tissues.

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$ 240.00
Cat# ABO12550
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