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Anti-SKAP55 Picoband Antibody

     
  • WB - Anti-SKAP55 Picoband Antibody ABO12576
    Western blot analysis of SKAP55 expression in PANC whole cell lysates (lane 1) and JURKAT whole cell lysates (lane 2). SKAP55 at 60KD was detected using rabbit anti- SKAP55 Antigen Affinity purified polyclonal antibody (Catalog # ABO12576) at0.5 μg/mL. The blot was developed using chemiluminescence (ECL) method .
    detail
  • IHC - Anti-SKAP55 Picoband Antibody ABO12576
    SKAP55 was detected in paraffin-embedded sections of rat spleen tissues using rabbit anti- SKAP55 Antigen Affinity purified polyclonal antibody (Catalog # ABO12576) at 1 ??g/mL. The immunohistochemical section was developed using SABC method .
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P
Primary Accession Q86WV1
Host Rabbit
Reactivity Human, Rat
Clonality Polyclonal
Format Lyophilized
Description Rabbit IgG polyclonal antibody for Src kinase-associated phosphoprotein 1(SKAP1) detection. Tested with WB, IHC-P in Human;Rat.
Reconstitution Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Additional Information
Gene ID 8631
Other Names Src kinase-associated phosphoprotein 1, Src family-associated phosphoprotein 1, Src kinase-associated phosphoprotein of 55 kDa, SKAP-55, pp55, SKAP1, SCAP1, SKAP55
Calculated MW 41432 MW KDa
Application Details Immunohistochemistry(Paraffin-embedded Section), 0.5-1 µg/ml, Rat, Human, By Heat

Western blot, 0.1-0.5 µg/ml, Human
Subcellular Localization Cytoplasm. Nucleus. Cell membrane. Upon T-cell stimulation, translocates to lipid rafts at the cell membrane.
Tissue Specificity Highly expressed in thymocytes and peripheral blood lymphocytes. Also expressed in spleen cells and testis. Present in T-cells (at protein level). .
Protein Name Src kinase-associated phosphoprotein 1
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Immunogen A synthetic peptide corresponding to a sequence in the middle region of human SKAP55 (163-189aa RMAPHLRRDSKKESCFELTSQDRRSYE), different from the related mouse and rat sequences by two amino acids.
Purification Immunogen affinity purified.
Cross Reactivity No cross reactivity with other proteins.
Storage At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Protein Information
Name SKAP1
Synonyms SCAP1, SKAP55
Function Positively regulates T-cell receptor signaling by enhancing the MAP kinase pathway. Required for optimal conjugation between T- cells and antigen-presenting cells by promoting the clustering of integrin ITGAL on the surface of T-cells. May be involved in high affinity immunoglobulin epsilon receptor signaling in mast cells.
Cellular Location Cytoplasm. Nucleus. Cell membrane. Note=Upon T- cell stimulation, translocates to lipid rafts at the cell membrane
Tissue Location Highly expressed in thymocytes and peripheral blood lymphocytes. Also expressed in spleen cells and testis. Present in T- cells (at protein level).
Research Areas
Citations (0)
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Background

Src kinase-associated phosphoprotein 1 is an adapter protein that in humans is encoded by the SKAP1 gene. This gene encodes a T cell adaptor protein, a class of intracellular molecules with modular domains capable of recruiting additional proteins but that exhibit no intrinsic enzymatic activity. The encoded protein contains a unique N-terminal region followed by a PH domain and C-terminal SH3 domain. Along with the adhesion and degranulation-promoting adaptor protein, the encoded protein plays a critical role in inside-out signaling by coupling T-cell antigen receptor stimulation to the activation of integrins.

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$ 280.00
Cat# ABO12576
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