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Anti-Caldesmon Picoband Antibody

     
  • WB - Anti-Caldesmon Picoband Antibody ABO12607
    Western blot analysis of Caldesmon expression in rat skeletal muscle extract (lane 1) and HELA whole cell lysates (lane 2). Caldesmon at 93KD was detected using rabbit anti- Caldesmon Antigen Affinity purified polyclonal antibody (Catalog # ABO12607) at 0.5 μg/mL. The blot was developed using chemiluminescence (ECL) method .
  • IHC - Anti-Caldesmon Picoband Antibody ABO12607
    Caldesmon was detected in paraffin-embedded sections of mouse intestine tissues using rabbit anti- Caldesmon Antigen Affinity purified polyclonal antibody (Catalog # ABO12607) at 1 μg/mL. The immunohistochemical section was developed using SABC method .
  • IHC - Anti-Caldesmon Picoband Antibody ABO12607
    Caldesmon was detected in paraffin-embedded sections of rat intestine tissues using rabbit anti- Caldesmon Antigen Affinity purified polyclonal antibody (Catalog # ABO12607) at 1 μg/mL. The immunohistochemical section was developed using SABC method .
  • IHC - Anti-Caldesmon Picoband Antibody ABO12607
    Caldesmon was detected in paraffin-embedded sections of human intestinal tissues using rabbit anti- Caldesmon Antigen Affinity purified polyclonal antibody (Catalog # ABO12607) at 1 ??g/mL. The immunohistochemical section was developed using SABC method .
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P
Primary Accession Q05682
Host Rabbit
Reactivity Human, Mouse, Rat
Clonality Polyclonal
Format Lyophilized
Description Rabbit IgG polyclonal antibody for Caldesmon(CALD1) detection. Tested with WB, IHC-P in Human;Mouse;Rat.
Reconstitution Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Additional Information
Gene ID 800
Other Names Caldesmon, CDM, CALD1, CAD, CDM
Calculated MW 93231 MW KDa
Application Details Immunohistochemistry(Paraffin-embedded Section), 0.5-1 µg/ml, Human, Mouse, Rat, By Heat

Western blot, 0.1-0.5 µg/ml, Human, Rat
Subcellular Localization Cytoplasm, cytoskeleton . Cytoplasm, myofibril . On thin filaments in smooth muscle and on stress fibers in fibroblasts (nonmuscle). .
Tissue Specificity High-molecular-weight caldesmon (isoform 1) is predominantly expressed in smooth muscles, whereas low-molecular- weight caldesmon (isoforms 2, 3, 4 and 5) are widely distributed in non-muscle tissues and cells. Not expressed in skeletal muscle or heart.
Protein Name Caldesmon
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Immunogen E. coli-derived human Caldesmon recombinant protein (Position: M1-E120). Human Caldesmon shares 87.6% amino acid (aa) sequence identity with rat Caldesmon.
Purification Immunogen affinity purified.
Cross Reactivity No cross reactivity with other proteins
Storage At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Protein Information
Name CALD1
Synonyms CAD, CDM
Function Actin- and myosin-binding protein implicated in the regulation of actomyosin interactions in smooth muscle and nonmuscle cells (could act as a bridge between myosin and actin filaments). Stimulates actin binding of tropomyosin which increases the stabilization of actin filament structure. In muscle tissues, inhibits the actomyosin ATPase by binding to F-actin. This inhibition is attenuated by calcium-calmodulin and is potentiated by tropomyosin. Interacts with actin, myosin, two molecules of tropomyosin and with calmodulin. Also play an essential role during cellular mitosis and receptor capping. Involved in Schwann cell migration during peripheral nerve regeneration (By similarity).
Cellular Location Cytoplasm, cytoskeleton {ECO:0000250|UniProtKB:P13505}. Cytoplasm, myofibril {ECO:0000250|UniProtKB:P13505}. Cytoplasm, cytoskeleton, stress fiber {ECO:0000250|UniProtKB:P13505}. Note=On thin filaments in smooth muscle and on stress fibers in fibroblasts (nonmuscle) {ECO:0000250|UniProtKB:P13505}
Tissue Location High-molecular-weight caldesmon (isoform 1) is predominantly expressed in smooth muscles, whereas low-molecular- weight caldesmon (isoforms 2, 3, 4 and 5) are widely distributed in non-muscle tissues and cells. Not expressed in skeletal muscle or heart
Research Areas
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Background

Caldesmon is a protein that in humans is encoded by the CALD1 gene. It is mapped to 7q33. This gene encodes a calmodulin- and actin-binding protein that plays an essential role in the regulation of smooth muscle and nonmuscle contraction. The conserved domain of this protein possesses the binding activities to Ca(2+)-calmodulin, actin, tropomyosin, myosin, and phospholipids. This protein is a potent inhibitor of the actin-tropomyosin activated myosin MgATPase, and serves as a mediating factor for Ca(2+)-dependent inhibition of smooth muscle contraction. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms.

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$ 280.00
Cat# ABO12607
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