|Application ||WB, IHC-P|
|Description||Rabbit IgG polyclonal antibody for T-cell surface antigen CD2(CD2) detection. Tested with WB, IHC-P in Mouse.|
|Reconstitution||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Other Names||T-cell surface antigen CD2, LFA-2, LFA-3 receptor, Lymphocyte antigen 37, Ly-37, T-cell surface antigen T11/Leu-5, CD2, Cd2, Ly-37|
|Calculated MW||38415 MW KDa|
|Application Details||Immunohistochemistry(Paraffin-embedded Section), 0.5-1 µg/ml, Mouse, By Heat|
Western blot, 0.1-0.5 µg/ml, Mouse
|Subcellular Localization||Membrane; Single-pass type I membrane protein.|
|Protein Name||T-cell surface antigen CD2|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.|
|Immunogen||E. coli-derived mouse CD2 recombinant protein (Position: R23-S203). Mouse CD2 shares 49.7% and 73.3% amino acid (aa) sequence identity with human and rat CD2, respectively.|
|Purification||Immunogen affinity purified.|
|Cross Reactivity||No cross reactivity with other proteins|
|Storage||At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
|Function||CD2 interacts with lymphocyte function-associated antigen CD58 (LFA-3) and CD48/BCM1 to mediate adhesion between T- cells and other cell types. CD2 is implicated in the triggering of T-cells, the cytoplasmic domain is implicated in the signaling function.|
|Cellular Location||Cell membrane; Single-pass type I membrane protein|
|Tissue Location||Detected in thymus and spleen.|
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Provided below are standard protocols that you may find useful for product applications.
CD2 (cluster of differentiation 2) is a cell adhesion molecule found on the surface of T cells and natural killer (NK) cells. It has also been called T-cell surface antigen T11/Leu-5, LFA-2, LFA-3 receptor, erythrocyte receptor and rosette receptor. Monoclonal antibodies directed against CD2 inhibit the formation of rosettes with sheep erythrocytes, indicating that CD2 is the erythrocyte receptor or is closely associated with it. CD2 is one of the earliest T-cell markers, being present on more than 95% of thymocytes. Due to its structural characteristics, CD2 is a member of the immunoglobulin superfamily; it possesses two immunoglobulin-like domains in its extracellular portion. The localization of CD2 to 1p13 is established by in situ hybridization. CD2 interacts with other adhesion molecules, such as lymphocyte function-associated antigen-3 (LFA-3/CD58) in humans, or CD48 in rodents, which are expressed on the surfaces of other cells. With the use of transgenic mice, such an LCR is identified within the 3-prime flanking region of the human CD2 gene.
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