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Anti-IL-3 Antibody

     
  • WB - Anti-IL-3 Antibody ABO12730
    Anti- IL-3 antibody, ABO12730, Western blottingAll lanes: Anti IL-3 (ABO12730) at 0.5ug/mlWB: Recombinant Rat IL-3 Protein 0.5ngPredicted bind size: 15KDObserved bind size: 15KD
  • WB - Anti-IL-3 Antibody ABO12730
    Anti- IL-3 antibody, ABO12730, Western blottingAll lanes: Anti IL-3 (ABO12730) at 0.5ug/mlLane 1: PC-12 Whole Cell Lysate at 40ugLane 2: NRK Whole Cell Lysate at 40ugLane 3: RH35 Whole Cell Lysate at 40ugPredicted bind size: 17KDObserved bind size: 37KD
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession P04823
Host Rabbit
Reactivity Rat
Clonality Polyclonal
Format Lyophilized
Description Rabbit IgG polyclonal antibody for Interleukin-3(IL3) detection. Tested with WB, ELISA in Rat.
Reconstitution Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Additional Information
Other Names Interleukin-3, IL-3, Hematopoietic growth factor, Mast cell growth factor, MCGF, Multipotential colony-stimulating factor, P-cell-stimulating factor, Il3, Il-3
Calculated MW 18631 MW KDa
Application Details ELISA , 0.1-0.5 µg/ml, Rat, -
Western blot, 0.1-0.5 µg/ml, Rat
Subcellular Localization Secreted.
Tissue Specificity Activated T-cells, mast cells, natural killer cells.
Protein Name Interleukin-3
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Immunogen E. coli-derived rat IL-3 recombinant protein (Position: S28-C166). Rat IL-3 shares 32% and 55% amino acid (aa) sequence identity with human and mouse IL-3, respectively.
Purification Immunogen affinity purified.
Cross Reactivity No cross reactivity with other proteins
Storage At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Protein Information
Name Il3
Synonyms Il-3
Function Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages.
Cellular Location Secreted.
Tissue Location Activated T-cells, mast cells, natural killer cells
Citations (0)

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Background

Interleukin 3, also known as IL-3, is a protein that in humans is encoded by the IL3 gene. It is mapped to 5q31.1. IL-3 is an interleukin, a type of biological signal (cytokine) that can improve the body's natural response to disease as part of the immune system. It acts by binding to the interleukin-3 receptor. IL-3 stimulates the differentiation of multipotent hematopoietic stem cells into myeloid progenitor cells or, with the addition of IL-7, into lymphoid progenitor cells. In addition, IL-3 stimulates proliferation of all cells in the myeloid lineage (granulocytes, monocytes, and dendritic cells), in conjunction with other cytokines, e.g., Erythropoietin (EPO), Granulocyte macrophage colony-stimulating factor (GM-CSF), and IL-6. IL-3 is secreted by basophils and activated T cells to support growth and differentiation of T cells from the bone marrow in an immune response.

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$ 240.00
Cat# ABO12730
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