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Anti-Occludin Antibody

     
  • WB - Anti-Occludin Antibody ABO12740
    Anti- Occludin antibody, ABO12740, Western blottingAll lanes: Anti Occludin (ABO12740) at 0.5ug/mlLane 1: SW620 Whole Cell Lysate at 40ugLane 2: COLO320 Whole Cell Lysate at 40ugLane 3: A549 Whole Cell Lysate at 40ugLane 4: 293T Whole Cell Lysate at 40ugLane 5: U87 Whole Cell Lysate at 40ugPredicted bind size: 59KDObserved bind size: 59KD
  • IHC - Anti-Occludin Antibody ABO12740
    Anti- Occludin antibody, ABO12740,IHC(P)IHC(P): Human Mammary Cancer Tissue
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P
Primary Accession Q16625
Host Rabbit
Reactivity Human
Clonality Polyclonal
Format Lyophilized
Description Rabbit IgG polyclonal antibody for Occludin(OCLN) detection. Tested with WB, IHC-P in Human.
Reconstitution Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Additional Information
Gene ID 100506658
Other Names Occludin, OCLN
Calculated MW 59144 MW KDa
Application Details Immunohistochemistry(Paraffin-embedded Section), 0.5-1 µg/ml, Human, By Heat
Western blot, 0.1-0.5 µg/ml, Human
Subcellular Localization Membrane; Multi-pass membrane protein. Cell junction, tight junction.
Tissue Specificity Localized at tight junctions of both epithelial and endothelial cells. Highly expressed in kidney. Not detected in testis.
Protein Name Occludin
Contents Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Immunogen E.coli-derived human Occludin recombinant protein (Position: N373-T522). Human Occludin shares 89% amino acid (aa) sequence identity with both mouse and rat Occludin.
Purification Immunogen affinity purified.
Cross Reactivity No cross reactivity with other proteins
Storage At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Sequence Similarities Belongs to the ELL/occludin family.
Protein Information
Name OCLN
Function May play a role in the formation and regulation of the tight junction (TJ) paracellular permeability barrier. It is able to induce adhesion when expressed in cells lacking tight junctions.
Cellular Location Cell membrane; Multi- pass membrane protein. Cell junction, tight junction
Tissue Location Localized at tight junctions of both epithelial and endothelial cells. Highly expressed in kidney. Not detected in testis
Research Areas
Citations (0)

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Background

Occludin is an integral membrane protein that is located at tight junctions. It is a member of a family of proteins containing the highly conserved Marvel domain, which contains 4 transmembrane-helix regions. This gene is mapped to 5q13.2. Occludin regulates TGF-beta receptor type I localization for efficient TGF-beta-dependent dissolution of tight junctions during epithelial-mesenchymal transitions. Human Occludin is an essential hepatitis C virus (HCV) cell entry factor that is able to render murine cells infectable with HCV glycoproteins. It has been found that occludin is involved in cell migration and functions to recruit active PI3 kinase to the leading edge, resulting in RAC1 activation and formation of lamellipodia.

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$ 280.00
Cat# ABO12740
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