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>   home   >   Products   >   Primary Antibodies   >   Immunology   >   Anti-LFA3 Antibody   

Anti-LFA3 Antibody

     
  • WB - Anti-LFA3 Antibody ABO12782
    Western blot analysis of LFA3 expression in HELA whole cell lysates (lane 1) and K562 whole cell lysates (lane 2). LFA3 at 35KD was detected using rabbit anti- LFA3 Antigen Affinity purified polyclonal antibody (Catalog # ABO12782) at 0.5 ??g/mL. The blot was developed using chemiluminescence (ECL) method .
  • IHC - Anti-LFA3 Antibody ABO12782
    LFA3 was detected in paraffin-embedded sections of human tonsil tissues using rabbit anti- LFA3 Antigen Affinity purified polyclonal antibody (Catalog # ABO12782) at 1 μg/mL. The immunohistochemical section was developed using SABC method .
  • FC - Anti-LFA3 Antibody ABO12782
    Figure 3. Flow Cytometry analysis of Hela cells using anti-LFA3 antibody (ABO12782).Overlay histogram showing Hela cells stained with ABO12782 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-LFA3 Antibody (ABO12782,1μg/1x106 cells) for 30 min at 20°C. DyLight?488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IHC-F, FC, ICC
Primary Accession P19256
Host Rabbit
Reactivity Human
Clonality Polyclonal
Format Lyophilized
Description Rabbit IgG polyclonal antibody for Lymphocyte function-associated antigen 3(CD58) detection. Tested with WB, IHC-P, IHC-F, ICC, FCM in Human.
Reconstitution Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Additional Information
Gene ID 965
Other Names Lymphocyte function-associated antigen 3, Ag3, Surface glycoprotein LFA-3, CD58, CD58, LFA3
Calculated MW 28147 MW KDa
Application Details Immunohistochemistry(Paraffin-embedded Section), 0.5-1 µg/ml, By Heat
Immunohistochemistry(Frozen Section), 0.5-1 µg/ml

Immunocytochemistry, 0.5-1 µg/ml

Western blot, 0.1-0.5 µg/ml
Flow Cytometry, 1-3μg/1x106cells
Subcellular Localization Isoform 1: Cell membrane; Single-pass type I membrane protein.
Protein Name Lymphocyte function-associated antigen 3
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Immunogen E. coli-derived human LFA3 recombinant protein (Position: F29-R215).
Purification Immunogen affinity purified.
Cross Reactivity No cross reactivity with other proteins
Storage At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Protein Information
Name CD58
Synonyms LFA3
Function Ligand of the T-lymphocyte CD2 glycoprotein. This interaction is important in mediating thymocyte interactions with thymic epithelial cells, antigen-independent and -dependent interactions of T-lymphocytes with target cells and antigen- presenting cells and the T-lymphocyte rosetting with erythrocytes. In addition, the LFA-3/CD2 interaction may prime response by both the CD2+ and LFA-3+ cells.
Cellular Location Isoform 1: Cell membrane; Single-pass type I membrane protein
Research Areas
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Background

CD58, or lymphocyte function-associated antigen 3 (LFA-3), is a cell adhesion molecule expressed on Antigen Presenting Cells (APC), particularly macrophages. It binds to CD2 (LFA-2) on T cells and is important in strengthening the adhesion between the T cells and Professional Antigen Presenting Cells. This adhesion occurs as part of the transitory initial encounters between T cells and Antigen Presenting Cells before T cell activation, when T cells are roaming the lymph nodes looking at the surface of APCs for peptide:MHC complexes the T-cell receptors are reactive to. The LFA3 gene is mapped to chromosome 1p13, which is the same location as CD2.

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$ 280.00
Cat# ABO12782
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