|Application ||WB, IF|
|Reactivity||Human, Mouse, Dog|
|Calculated MW||23279 Da|
|Application & Usage||Western blot analysis (1-4 µg/ml). The antibody detects 28 kDa protein, corresponding to the apparent molecular weight of FADD in SDS-PAGE However, the optimal conditions should be determined individually. HeLa cell lysate can be used as a positive control.immunoblots from samples of human and mouse origins.|
|Other Names||FAS-associated death domain protein, GIG3, MGC8528, MORT1|
|Formulation||100 µg (200 µg/ml) affinity purified antibody in PBS containing 30% glycerol, 0.5% BSA and 0.01% thimerosal|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||FADD Antibody (Clone T1B) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Apoptotic adaptor molecule that recruits caspase-8 or caspase-10 to the activated Fas (CD95) or TNFR-1 receptors. The resulting aggregate called the death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation. Active caspase-8 initiates the subsequent cascade of caspases mediating apoptosis. Involved in interferon-mediated antiviral immune response, playing a role in the positive regulation of interferon signaling.|
|Tissue Location||Expressed in a wide variety of tissues, except for peripheral blood mononuclear leukocytes|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
email@example.com, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
Fas-associating death domain containing protein (FADD), also known as mediator of receptor induced toxicity (MORT1), is a 24 kDa cytosolic protein. The protein is involved in both Fas and TNF-R induced cell death pathway. FADD specifically binds to Fas, an association mediated by their homologous death domains. Overexpression of FADD induces apoptosis that can be inhibited by CrmA, a poxvirus protein that blocks both Fas- and TNF-induced cell death.
If you have used an Abgent product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at firstname.lastname@example.org.