FADD Antibody (Clone T1B)
Mouse Monoclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, IF |
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Primary Accession | Q13158 |
Other Accession | NM_003824 |
Reactivity | Human, Mouse, Dog |
Host | Mouse |
Clonality | Monoclonal |
Isotype | Mouse IgG1 |
Clone Names | T1B |
Calculated MW | 23279 Da |
Gene ID | 8772 |
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Application & Usage | Western blot analysis (1-4 µg/ml). The antibody detects 28 kDa protein, corresponding to the apparent molecular weight of FADD in SDS-PAGE However, the optimal conditions should be determined individually. HeLa cell lysate can be used as a positive control.immunoblots from samples of human and mouse origins. |
Other Names | FAS-associated death domain protein, GIG3, MGC8528, MORT1 |
Target/Specificity | FADD |
Antibody Form | Liquid |
Appearance | Colorless liquid |
Formulation | 100 µg (200 µg/ml) affinity purified antibody in PBS containing 30% glycerol, 0.5% BSA and 0.01% thimerosal |
Handling | The antibody solution should be gently mixed before use. |
Reconstitution & Storage | -20 °C |
Background Descriptions | |
Precautions | FADD Antibody (Clone T1B) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | FADD {ECO:0000303|PubMed:7538907, ECO:0000312|HGNC:HGNC:3573} |
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Function | Apoptotic adaptor molecule that recruits caspase-8 or caspase-10 to the activated Fas (CD95) or TNFR-1 receptors (PubMed:7538907, PubMed:23955153, PubMed:19118384, PubMed:20935634, PubMed:16762833, PubMed:24025841). The resulting aggregate called the death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation (PubMed:7538907, PubMed:19118384, PubMed:20935634, PubMed:16762833). Active caspase-8 initiates the subsequent cascade of caspases mediating apoptosis (PubMed:16762833). Involved in interferon- mediated antiviral immune response, playing a role in the positive regulation of interferon signaling (PubMed:21109225). |
Tissue Location | Expressed in a wide variety of tissues, except for peripheral blood mononuclear leukocytes. |
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Provided below are standard protocols that you may find useful for product applications.
Background
Fas-associating death domain containing protein (FADD), also known as mediator of receptor induced toxicity (MORT1), is a 24 kDa cytosolic protein. The protein is involved in both Fas and TNF-R induced cell death pathway. FADD specifically binds to Fas, an association mediated by their homologous death domains. Overexpression of FADD induces apoptosis that can be inhibited by CrmA, a poxvirus protein that blocks both Fas- and TNF-induced cell death.
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