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Apaf-1 Antibody

Rabbit Polyclonal Antibody

  • WB - Apaf-1 Antibody ABV10011
    Western blot analysis of Apaf1 expression in human heart tissue lysate.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession O14727
Reactivity Human, Mouse, Rat, Rabbit, Monkey, Bovine, Horse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 141840 Da
Additional Information
Gene ID 317
Application & Usage Western blotting (0.5-4 µg/ml). However, the optimal conditions should be determined individually. The immunoaffinity purified antibody detects a 130 kDa protein, corresponding to the apparent molecular weight of Apaf-1 on SDS-PAGE immunoblots.
Other Names APAF1, CED4, DKFZp781B1145, KIAA0413
Target/Specificity Apaf-1
Antibody Form Liquid
Appearance Colorless liquid
Formulation 100 µg (0.2 mg/ml) immunoaffinity purified, rabbit anti-Apaf-1 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.05% BSA, 0.01% thimerosal.
Handling The antibody solution should be gently mixed before use.
Reconstitution & Storage -20 °C
Background Descriptions
PrecautionsApaf-1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name APAF1
Synonyms KIAA0413
Function Oligomeric Apaf-1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis. This activation requires ATP. Isoform 6 is less effective in inducing apoptosis.
Cellular Location Cytoplasm.
Tissue Location Ubiquitous. Highest levels of expression in adult spleen and peripheral blood leukocytes, and in fetal brain, kidney and lung. Isoform 1 is expressed in heart, kidney and liver
Research Areas
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During apoptosis, Apaf-1 (apoptosis protease activating factor 1) binds to cytochrome c (Apaf-2), a reaction that requires dATP, and this leads to the formation of a large multimeric complex. The complex recruits pro-caspase-9, and activates caspase-9 (Apaf-3). Activated caspase-9 in turn cleaves and activates caspase-3, one of the proteases responsible for the proteolytic cleavage of many key proteins in apoptosis. Apaf-1 is ubiquitously expressed in human tissues.

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$ 265.00
Cat# ABV10011
(40 western blots)
Availability: 5-7days
Bulk Size
Seasonal Special on Bulk Order
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