|Application ||WB, IF, IP|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||45159 Da|
|Application & Usage||Western blot analysis (0.5-4 µg/ml), immunoprecipitation (4-8 µg/ml) and immunofluorescence (10-20 µg/ml). The immunoaffinity purified antibody recognizes 45 kDa procaspase-1 and the 20 kDa cleaved product.|
|Other Names||CASP1, IL1BCE, P45, Interleukin-1 beta convertase, IL-1BC, IL1BC, p45, IL1B-convertase, CASP-1, ICE|
|Formulation||100 µg (0.2 mg/ml) affinity purified rabbit anti-caspase-1 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||Caspase-1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Thiol protease that cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes. Important for defense against pathogens. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Can also promote apoptosis. Upon inflammasome activation, during DNA virus infection but not RNA virus challenge, controls antiviral immunity through the cleavage of CGAS, rendering it inactive (PubMed:28314590).|
|Tissue Location||Expressed in larger amounts in spleen and lung. Detected in liver, heart, small intestine, colon, thymus, prostate, skeletal muscle, peripheral blood leukocytes, kidney and testis. No expression in the brain.|
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Provided below are standard protocols that you may find useful for product applications.
Caspase family of cysteine proteases has been shown to play a key role in apoptosis. Caspase-1 (ICE, interleukin-1β-converting enzyme) is the mammalian caspase responsible for the proteolytic conversion of the proforms of interleukin-1β and IL-18 into active cytokines. Like other caspases, Caspase-1 itself is synthesized as a pro-enzyme that is cleaved during activation into a large (20 kDa) and small (10 kDa) subunits. The cellular role of caspase-1 appears limited to cytokine activation as they do not have obvious roles in apoptosis.
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