|Application ||WB, IP|
|Application & Usage||Western blotting (0.5 µg/ml).|
|Other Names||FLAG, DYKDDDDK|
|Formulation||100 µg (0.5 mg/ml) peptide affinity purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 0.5% BSA, and 0.01% thimerosal.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||DYKDDDDK Tag Antibody (Clone IE6) is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
The DYKDDDDK Tag Antibody is a high-affinity mouse monoclonal antibody that can be used to detect DYKDDDDK-tagged proteins. It can detect N-terminal, C-terminal and internal tagged fusion proteins. This antibody recognizes the same epitope recognized by Sigma’s anti-FLAG® antibodies.
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