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Cytochrome c Antibody

Rabbit Polyclonal Antibody

  • WB - Cytochrome c Antibody ABV10017
    Western blot analysis of cytochrome c in Jurkat (left lane) and 3T3 (right lane) cell lysates.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P10715
Other Accession NP_036972
Reactivity Human, Mouse, Rat, Chicken, Bovine, Dog
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 11743 Da
Additional Information
Gene ID 25310
Application & Usage Western blotting (0.5-4 µg/ml) and immunoprecipitation (10-20 µg/ml). The cytochrome c antibody detects the 12.6 kDa cytochrome c from human, mouse, and rat samples. Jurkat cell lysate, NIH3T3 cell lysate, and rat kidney tissue lysate can be used as positive controls.
Other Names CYCS, CYC
Target/Specificity Cytochrome c
Antibody Form Liquid
Appearance Colorless liquid
Formulation 100 µg (0.2 mg/ml) affinity purified rabbit anti-Cytochrome c polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.
Handling The antibody solution should be gently mixed before use.
Reconstitution & Storage -20 °C
Background Descriptions
PrecautionsCytochrome c Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name Cyct
Function Electron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain.
Cellular Location Mitochondrion intermembrane space. Note=Loosely associated with the inner membrane
Tissue Location This is one of two isocytochromes C found in the testis. The other is identical with the form found in other rat tissues. These cytochromes are assumed to be located in the sperm
Citations (0)

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Cytochrome c (m.w. 12,500) is an electron transport protein from mitochondria. It is released from mitochondria to cytoplasm during the early stages of apoptosis, prior to caspase activation, DNA fragmentation, and loss of membrane potential. The cytoplasmic cytochrome c is associated with Apaf-1 and caspase-9 to activate caspase-3 and other caspases

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$ 265.00
$ 89.00
Cat# ABV10017
(40 western blots)
Availability: 5-7days
Bulk Size
Seasonal Special on Bulk Order
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