|Application ||WB, IP|
|Reactivity||Human, Mouse, Rat, Chicken, Bovine, Dog|
|Calculated MW||11743 Da|
|Application & Usage||Western blotting (0.5-4 µg/ml) and immunoprecipitation (10-20 µg/ml). The cytochrome c antibody detects the 12.6 kDa cytochrome c from human, mouse, and rat samples. Jurkat cell lysate, NIH3T3 cell lysate, and rat kidney tissue lysate can be used as positive controls.|
|Other Names||CYCS, CYC|
|Formulation||100 µg (0.2 mg/ml) affinity purified rabbit anti-Cytochrome c polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||Cytochrome c Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Electron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain.|
|Cellular Location||Mitochondrion intermembrane space. Note=Loosely associated with the inner membrane|
|Tissue Location||This is one of two isocytochromes C found in the testis. The other is identical with the form found in other rat tissues. These cytochromes are assumed to be located in the sperm|
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Provided below are standard protocols that you may find useful for product applications.
Cytochrome c (m.w. 12,500) is an electron transport protein from mitochondria. It is released from mitochondria to cytoplasm during the early stages of apoptosis, prior to caspase activation, DNA fragmentation, and loss of membrane potential. The cytoplasmic cytochrome c is associated with Apaf-1 and caspase-9 to activate caspase-3 and other caspases
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