|Reactivity||Human, Mouse, Rat|
|Application & Usage||Western blot analysis (0.5-4 µg/ml). However, the optimal conditions should be determined individually. The antibody detects a 12.0 kDa protein corresponding to the apparent molecular weight of cytochrome c.|
|Other Names||CYCS, CYC|
|Formulation||100 µg (0.2 mg/ml) purified IgG in PBS containing 50% glycerol, 0.5 % BSA and 0.01% Thimerosal and 50% glycerol|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||Cytochrome c Antibody (Clone 7H8.2C12) is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
Cytochrome c (m.w. 12,500) is an electron transport protein from mitochondria. It is released from mitochondria to cytoplasm during the early stages of apoptosis, prior to caspase activation, DNA fragmentation, and loss of membrane potential. The cytoplasmic cytochrome c is associated with Apaf-1 and caspase-9 to activate caspase-3 and other caspases.
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