RAIDD Antibody
Rabbit Polyclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB |
---|---|
Primary Accession | P78560 |
Other Accession | AAH37905 |
Reactivity | Human, Mouse, Rat |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 22745 Da |
Gene ID | 8738 |
---|---|
Application & Usage | Western blot analysis (0.5-4 µg/ml). However, the optimal conditions should be determined individually. The antibody detects a 22 kDa band, corresponding to the expected molecular mass of RAIDD on immunoblots. |
Other Names | CRADD, MGC9163, |
Target/Specificity | RAIDD |
Antibody Form | Liquid |
Appearance | Colorless liquid |
Formulation | 100 µg (0.2 mg/ml) affinity purified rabbit anti-RAIDD polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal. |
Handling | The antibody solution should be gently mixed before use. |
Reconstitution & Storage | -20 °C |
Background Descriptions | |
Precautions | RAIDD Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | CRADD |
---|---|
Synonyms | RAIDD |
Function | Adapter protein that associates with PIDD1 and the caspase CASP2 to form the PIDDosome, a complex that activates CASP2 and triggers apoptosis (PubMed:9044836, PubMed:15073321, PubMed:16652156, PubMed:17159900, PubMed:17289572). Also recruits CASP2 to the TNFR-1 signaling complex through its interaction with RIPK1 and TRADD and may play a role in the tumor necrosis factor-mediated signaling pathway (PubMed:8985253). |
Cellular Location | Cytoplasm {ECO:0000250|UniProtKB:O88843}. Nucleus {ECO:0000250|UniProtKB:O88843} |
Tissue Location | Constitutively expressed in most tissues, with particularly high expression in adult heart, testis, liver, skeletal muscle, fetal liver and kidney. |
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Provided below are standard protocols that you may find useful for product applications.
Background
Caspase family of cysteine proteases plays a key role in apoptosis. Caspase-9 is one of the most important caspases among the caspase family members. Upon induction of apoptosis, cytochrome c released from mitochondria associates with procaspase-9/Apaf-1. The complex processes procaspase-9 into a large subunit (35 kDa or 17-25 kDa) and small (10 kDa) by self-cleavage at D315. Activated caspase-9 further cleaves other caspase members including caspase-3, one of the proteases responsible for the proteolytic cleavage of many key proteins in apoptosis. In addition to self-cleavage, procaspase-9 can also be cleaved in vivo by caspase-3 at D330. The process served as a positive feedback to amplify the apoptotic signal in caspase activation pathway.
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