FADD Antibody
Rabbit Polyclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, IP |
---|---|
Primary Accession | Q13158 |
Other Accession | NM_003824 |
Reactivity | Human, Mouse, Rat, Monkey |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 23279 Da |
Gene ID | 8772 |
---|---|
Application & Usage | Western blot analysis (0.5-4 µg/ml)and can also be used in immunoprecipitation. However, the optimal conditions should be determined individually. The antibody detects an ~28 kDa band, corresponding to the expected molecular mass of FADD on immunoblots. |
Other Names | FAS-associated death domain protein, GIG3, MGC8528, MORT1 |
Target/Specificity | FADD |
Antibody Form | Liquid |
Appearance | Colorless liquid |
Formulation | 100 µg (0.2 mg/ml) affinity purified rabbit anti-FADD polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal. |
Handling | The antibody solution should be gently mixed before use. |
Reconstitution & Storage | -20 °C |
Background Descriptions | |
Precautions | FADD Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | FADD {ECO:0000303|PubMed:7538907, ECO:0000312|HGNC:HGNC:3573} |
---|---|
Function | Apoptotic adaptor molecule that recruits caspase-8 or caspase-10 to the activated Fas (CD95) or TNFR-1 receptors (PubMed:7538907, PubMed:23955153, PubMed:19118384, PubMed:20935634, PubMed:16762833, PubMed:24025841). The resulting aggregate called the death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation (PubMed:7538907, PubMed:19118384, PubMed:20935634, PubMed:16762833). Active caspase-8 initiates the subsequent cascade of caspases mediating apoptosis (PubMed:16762833). Involved in interferon- mediated antiviral immune response, playing a role in the positive regulation of interferon signaling (PubMed:21109225). |
Tissue Location | Expressed in a wide variety of tissues, except for peripheral blood mononuclear leukocytes. |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
info@abcepta.com, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
Background
Fas-associating death domain containing protein (FADD), also known as mediator of receptor induced toxicity (MORT1), is a 23 kDa cytosolic protein with an apparent molecular weight of 28 kDa on SDS-PAGE. The protein is involved in both Fas and TNF-R induced cell death pathway. FADD specifically binds to Fas to initiate the apoptotic signalling cascade. Overexpression of FADD induces apoptosis.
If you have used an Abcepta product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at tech@abcepta.com.