|Calculated MW||23280 Da|
|Application & Usage||Western blot analysis (0.5-4 µg/ml).|
|Other Names||Bcl-2-related ovarian killer|
|Formulation||100 µg (0.2 mg/ml) peptide affinity purified rabbit anti-Bok polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 50% glycerol, 1% BSA, 0.02% thimerosal.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||Bok Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Induces apoptosis in a manner dependent on BAX and BAK.|
|Cellular Location||Endoplasmic reticulum membrane; Single-pass membrane protein. Golgi apparatus membrane; Single-pass membrane protein. Note=The transmembrane domain is necessary and sufficient for Golgi and ER targeting|
|Tissue Location||Expressed in brain, liver, appendix and lymphoid tissues.|
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Bok (Bcl-2-related ovarian killer) is a pro-apoptotic Bcl-2 family protein identified in the ovary based on its dimerization with the anti-apoptotic protein Mcl-1. In addition to the Bcl-2 homology (BH) domains 1 and 2 and the transmembrane sequence, Bok also has a BH3 domain believed to be important for dimerization with selective anti-apoptotic Bcl-2 proteins and cell killing. Bok interacts strongly with some (Mcl-1, BHRF1, and Bfl-1) but not other (Bcl-2, Bcl-xL, and Bcl-w) anti-apoptotic members. In addition, cell killing induced by Bok was suppressed following coexpression with Mcl-1 and BHRF1, but not with Bcl-2, further indicating that Bok heterodimerized only with selective anti-apoptotic Bcl-2 proteins. Bok was highly expressed in the ovary, testis and uterus.
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