|Application ||WB, IF, E, IP|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||66408 Da|
|Application & Usage||Western blotting (0.5-2 µg/ml), immunoprecipitation (5 µg/ml), immuno-fluorescence (10-15 µg/ml), and ELISA (0.2-1 µg/ml). However, the optimal conditions should be determined individually.|
|Other Names||Lamin-B1, LMNB1, LMN2, LMNB|
|Formulation||100 µg (200 µg/ml) protein G purified antibody in PBS containing 1 mg/ml BSA and 1.5 mM sodium azide and 50% glycerol.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||Lamin B1 Antibody (Clone ZL-5) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin.|
|Cellular Location||Nucleus inner membrane; Lipid-anchor; Nucleoplasmic side|
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Provided below are standard protocols that you may find useful for product applications.
Lamins are classified into A- and B- type isoforms. Specifically, four distinct lamin isoforms have been identified: A, B1, B2, and C. Lamin A and C are alternatively spliced products of a single gene, whereas lamin B1 and B2 are encoded by distinct genes. During mitosis, lamins are phosphorylated by p34cdc2 and solubilized prior to chromosome condensation and nuclear breakdown. A direct role for lamins in chromosome condensation is s µggested by the fact that lamin B binds to specific DNA sequence motifs termed matrix attachment regions (MARs). Mars mediate the interaction of chromatin with the nuclear matrix. Importantly, some of the same processes that involve mitotic chromosome condensation and nuclear envelop breakdown are also activated during apoptosis. It appears that chromosome condensation during apoptosis is accompanied by degradation of lamin protein. Lamin degradation occurs prior to DNA fragmentation and this degradation is useful as an apoptotic marker.
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