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>   home   >   Products   >   Primary Antibodies   >   Lamin B1 Antibody (Clone ZL-5)   

Lamin B1 Antibody (Clone ZL-5)

Mouse Monoclonal Antibody

Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P20700
Reactivity Human, Mouse, Rat
Host Mouse
Clonality Monoclonal
Isotype Mouse IgG1-Kappa
Clone Names ZL-5
Calculated MW 66408 Da
Additional Information
Gene ID 4001
Application & Usage Western blotting (0.5-2 µg/ml), immunoprecipitation (5 µg/ml), immuno-fluorescence (10-15 µg/ml), and ELISA (0.2-1 µg/ml). However, the optimal conditions should be determined individually.
Other Names Lamin-B1, LMNB1, LMN2, LMNB
Target/Specificity Lamin B1
Antibody Form Liquid
Appearance Colorless liquid
Formulation 100 µg (200 µg/ml) protein G purified antibody in PBS containing 1 mg/ml BSA and 1.5 mM sodium azide and 50% glycerol.
Handling The antibody solution should be gently mixed before use.
Reconstitution & Storage -20 °C
Background Descriptions
PrecautionsLamin B1 Antibody (Clone ZL-5) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name LMNB1
Synonyms LMN2, LMNB
Function Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin.
Cellular Location Nucleus inner membrane; Lipid-anchor; Nucleoplasmic side
Citations (0)

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Lamins are classified into A- and B- type isoforms. Specifically, four distinct lamin isoforms have been identified: A, B1, B2, and C. Lamin A and C are alternatively spliced products of a single gene, whereas lamin B1 and B2 are encoded by distinct genes. During mitosis, lamins are phosphorylated by p34cdc2 and solubilized prior to chromosome condensation and nuclear breakdown. A direct role for lamins in chromosome condensation is s µggested by the fact that lamin B binds to specific DNA sequence motifs termed matrix attachment regions (MARs). Mars mediate the interaction of chromatin with the nuclear matrix. Importantly, some of the same processes that involve mitotic chromosome condensation and nuclear envelop breakdown are also activated during apoptosis. It appears that chromosome condensation during apoptosis is accompanied by degradation of lamin protein. Lamin degradation occurs prior to DNA fragmentation and this degradation is useful as an apoptotic marker.

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$ 365.00
Cat# ABV10035
Availability: 5-7days
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