DFF45 Antibody
Rabbit Polyclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB |
---|---|
Primary Accession | O54786 |
Other Accession | AAH58213 |
Reactivity | Mouse, Rat |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 36572 Da |
Gene ID | 13347 |
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Application & Usage | Western blotting (0.5-4 µg/ml). However, the optimal conditions should be determined individually. The antibody detects the 45 kDa and 28 kDa proteins, corresponding to the apparent molecular weight of DFF45/ICADL, and ICADS in immunoblots. |
Other Names | DFFA, DFF-45, ICAD, DFF1 |
Target/Specificity | DFF45 |
Antibody Form | Liquid |
Appearance | Colorless liquid |
Formulation | 100 µg (0.2 mg/ml) immunoaffinity purified rabbit DFF45 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal. |
Handling | The antibody solution should be gently mixed before use. |
Reconstitution & Storage | -20 °C |
Background Descriptions | |
Precautions | DFF45 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | Dffa |
---|---|
Synonyms | Icad |
Function | Inhibitor of the caspase-activated DNase (DFF40). |
Cellular Location | Cytoplasm. |
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Provided below are standard protocols that you may find useful for product applications.
Background
Apoptosis occurs during normal cellular development and involves dramatic changes in cellular structure. Disruption of apoptosis may contribute to cancer as well as many other types of diseases. Recently, a human DNA fragmentation factor (DFF45) was identified. DFF is composed of two subunts: DFF40 and DFF45. Cleavage of DFF45, which is mediated by caspase-3, leads to DFF40’s activation as a nuclease. Activation of DFF40 can lead to DNA fragmentation, a hallmark of apoptosis.
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