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>   home   >   Products   >   Primary Antibodies   >   Cell Biology   >   DFF40 Antibody   

DFF40 Antibody

Rabbit Polyclonal Antibody

     
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB
Primary Accession O54788
Other Accession AAH53052
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Additional Information
Application & Usage Western blotting (0.5-4 µg/ml) and Immunohistochemistry (2-8 µg/ml). However, the optimal conditions should be determined individually. The antibody detects a 43 kDa DFF40/CAD of human, mouse and rat origins. Mouse kidney tissue lysate can be used as a positive control.
Other Names DFFB, CAD, DFF-40 , CPAN , DFF2
Target/Specificity DFF40
Antibody Form Liquid
Appearance Colorless liquid
Formulation 100 µg (0.2 mg/ml) immunoaffinity purified rabbit DFF40 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.
Handling The antibody solution should be gently mixed before use.
Reconstitution & Storage -20 °C
Background Descriptions
PrecautionsDFF40 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name Dffb
Synonyms Cad
Function Nuclease that induces DNA fragmentation and chromatin condensation during apoptosis. Degrades naked DNA and induces apoptotic morphology.
Cellular Location Cytoplasm. Nucleus. EMBL; AB009377; BAA24977.1; -; mRNA CCDS; CCDS19004.1; - RefSeq; NP_031885.3; NM_007859.4 UniGene; Mm.388918; - PDB; 1C9F; NMR; -; A=1-87 PDB; 1F2R; NMR; -; C=1-87 PDB; 1V0D; X-ray; 2.60 A; A=1-329 PDBsum; 1C9F; - PDBsum; 1F2R; - PDBsum; 1V0D; - ProteinModelPortal; O54788; - SMR; O54788; - BioGrid; 199222; 3 IntAct; O54788; 3 MINT; O54788; - STRING; 10090.ENSMUSP00000030893; - PhosphoSitePlus; O54788; - MaxQB; O54788; - PaxDb; O54788; - PRIDE; O54788; - Ensembl; ENSMUST00000030893; ENSMUSP00000030893; ENSMUSG00000029027 GeneID; 13368; - KEGG; mmu:13368; - UCSC; uc008wav.2; mouse CTD; 1677; - MGI; MGI:1196287; Dffb eggNOG; ENOG410IFTE; Eukaryota eggNOG; ENOG41102TS; LUCA GeneTree; ENSGT00390000014490; - HOGENOM; HOG000006502; - HOVERGEN; HBG003828; - InParanoid; O54788; - KO; K02311; - OMA; TRVRGYY; - OrthoDB; EOG091G0BE7; - PhylomeDB; O54788; - TreeFam; TF102022; - Reactome; R-MMU-211227; Activation of DNA fragmentation factor EvolutionaryTrace; O54788; - PRO; PR:O54788; - Proteomes; UP000000589; Chromosome 4 Bgee; ENSMUSG00000029027; - CleanEx; MM_DFFB; - ExpressionAtlas; O54788; baseline and differential Genevisible; O54788; MM GO; GO:0005829; C:cytosol; ISO:MGI GO; GO:0000790; C:nuclear chromatin; ISO:MGI GO; GO:0005730; C:nucleolus; ISO:MGI GO; GO:0005634; C:nucleus; ISO:MGI GO; GO:0004536; F:deoxyribonuclease activity; ISO:MGI GO; GO:0097718; F:disordered domain specific binding; ISO:MGI GO; GO:0003677; F:DNA binding; IDA:MGI GO; GO:0019899; F:enzyme binding; ISO:MGI GO; GO:0042802; F:identical protein binding; ISO:MGI GO; GO:0004518; F:nuclease activity; IDA:MGI GO; GO:0019904; F:protein domain specific binding; ISO:MGI GO; GO:0030263; P:apoptotic chromosome condensation; ISO:MGI GO; GO:0006309; P:apoptotic DNA fragmentation; ISO:MGI GO; GO:0006308; P:DNA catabolic process; ISO:MGI GO; GO:0051260; P:protein homooligomerization; ISO:MGI InterPro; IPR015311; Apoptosis_DFF40 InterPro; IPR003508; CIDE-N_dom Pfam; PF02017; CIDE-N; 1 Pfam; PF09230; DFF40; 1 SMART; SM00266; CAD; 1 PROSITE; PS51135; CIDE_N; 1 1: Evidence at protein level; 3D-structure; Apoptosis; Complete proteome; Cytoplasm; Hydrolase; Nuclease; Nucleus; Reference proteome CHAIN 1 344 DNA fragmentation factor subunit beta /FTId=PRO_0000144714 DOMAIN 7 83 CIDE-N. {ECO:0000255|PROSITE- ProRule:PRU00447} STRAND 9 15 {ECO:0000244|PDB:1C9F} STRAND 16 19 {ECO:0000244|PDB:1F2R} STRAND 23 28 {ECO:0000244|PDB:1C9F} HELIX 29 39 {ECO:0000244|PDB:1C9F} STRAND 48 51 {ECO:0000244|PDB:1C9F} TURN 52 55 {ECO:0000244|PDB:1C9F} TURN 60 62 {ECO:0000244|PDB:1F2R} STRAND 70 75 {ECO:0000244|PDB:1C9F} HELIX 88 94 {ECO:0000244|PDB:1V0D} HELIX 103 111 {ECO:0000244|PDB:1V0D} HELIX 116 129 {ECO:0000244|PDB:1V0D} TURN 138 140 {ECO:0000244|PDB:1V0D} TURN 142 147 {ECO:0000244|PDB:1V0D} HELIX 155 175 {ECO:0000244|PDB:1V0D} HELIX 176 180 {ECO:0000244|PDB:1V0D} TURN 183 185 {ECO:0000244|PDB:1V0D} HELIX 186 202 {ECO:0000244|PDB:1V0D} HELIX 203 210 {ECO:0000244|PDB:1V0D} STRAND 212 214 {ECO:0000244|PDB:1V0D} TURN 216 218 {ECO:0000244|PDB:1V0D} STRAND 235 237 {ECO:0000244|PDB:1V0D} HELIX 246 248 {ECO:0000244|PDB:1V0D} HELIX 250 255 {ECO:0000244|PDB:1V0D} TURN 256 258 {ECO:0000244|PDB:1V0D} STRAND 259 265 {ECO:0000244|PDB:1V0D} TURN 267 270 {ECO:0000244|PDB:1V0D} HELIX 271 281 {ECO:0000244|PDB:1V0D} HELIX 287 295 {ECO:0000244|PDB:1V0D} TURN 297 299 {ECO:0000244|PDB:1V0D} STRAND 300 303 {ECO:0000244|PDB:1V0D} HELIX 305 307 {ECO:0000244|PDB:1V0D} TURN 319 321 {ECO:0000244|PDB:1V0D} SEQUENCE 344 AA; 39449 MW; E854B413EA139DE1 CRC64; MCAVLRQPKC VKLRALHSAC KFGVAARSCQ ELLRKGCVRF QLPMPGSRLC LYEDGTEVTD DCFPGLPNDA ELLLLTAGET WHGYVSDITR FLSVFNEPHA GVIQAARQLL SDEQAPLRQK LLADLLHHVS QNITAETREQ DPSWFEGLES RFRNKSGYLR YSCESRIRGY LREVSAYTSM VDEAAQEEYL RVLGSMCQKL KSVQYNGSYF DRGAEASSRL CTPEGWFSCQ GPFDLESCLS KHSINPYGNR ESRILFSTWN LDHIIEKKRT VVPTLAEAIQ DGREVNWEYF YSLLFTAENL KLVHIACHKK TTHKLECDRS RIYRPQTGSR RKQPARKKRP ARKR
Research Areas
Citations (0)

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Background

DFF is composed of two subunits: DFF40/CAD and DFF45/ICAD. Human DFF45 and its mouse homologue ICAD are the inhibitors of DFF40 and CAD, respectively. Cleavage of DFF45, which is mediated by caspase-3, leads to DFF40’s activation as a nuclease. Activation of DFF40/CAD can lead to DNA fragmentation, a hallmark of apoptosis. DFF40 mRNA is expressed in limited number of human tissues: pancreas, spleen, prostate, and ovary.

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$ 265.00
Cat# ABV10037-100
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