DFF40 Antibody
Rabbit Polyclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB |
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Primary Accession | O54788 |
Other Accession | AAH53052 |
Reactivity | Human, Mouse, Rat |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 39449 Da |
Gene ID | 13368 |
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Application & Usage | Western blotting (0.5-4 µg/ml) and Immunohistochemistry (2-8 µg/ml). However, the optimal conditions should be determined individually. The antibody detects a 43 kDa DFF40/CAD of human, mouse and rat origins. Mouse kidney tissue lysate can be used as a positive control. |
Other Names | DFFB, CAD, DFF-40 , CPAN , DFF2 |
Target/Specificity | DFF40 |
Antibody Form | Liquid |
Appearance | Colorless liquid |
Formulation | 100 µg (0.2 mg/ml) immunoaffinity purified rabbit DFF40 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal. |
Handling | The antibody solution should be gently mixed before use. |
Reconstitution & Storage | -20 °C |
Background Descriptions | |
Precautions | DFF40 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | Dffb |
---|---|
Synonyms | Cad |
Function | Nuclease that induces DNA fragmentation and chromatin condensation during apoptosis. Degrades naked DNA and induces apoptotic morphology. |
Cellular Location | Cytoplasm. Nucleus. |
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Provided below are standard protocols that you may find useful for product applications.
Background
DFF is composed of two subunits: DFF40/CAD and DFF45/ICAD. Human DFF45 and its mouse homologue ICAD are the inhibitors of DFF40 and CAD, respectively. Cleavage of DFF45, which is mediated by caspase-3, leads to DFF40’s activation as a nuclease. Activation of DFF40/CAD can lead to DNA fragmentation, a hallmark of apoptosis. DFF40 mRNA is expressed in limited number of human tissues: pancreas, spleen, prostate, and ovary.
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