|Reactivity||Human, Mouse, Rat|
|Calculated MW||66051 Da|
|Positive Control||Jurkat cell lysates|
|Application & Usage||The antibody can be used in Western Blot analysis (0.5-4 µg/ml). However, the optimal concentrations should be determined individually.|
|Other Names||MBD4, Methyl-CpG-binding domain protein 4, Methyl-CpG-binding endonuclease 1, Methyl-CpG-binding protein, MBD4, Mismatch-specific DNA N-glycosylase, MED1|
|Formulation||100 µg (0.5 mg/ml) affinity purified rabbit anti-MBD4 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 5 mM EDTA and 0.01% thimerosal.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||MBD4 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Mismatch-specific DNA N-glycosylase involved in DNA repair. Has thymine glycosylase activity and is specific for G:T mismatches within methylated and unmethylated CpG sites. Can also remove uracil or 5-fluorouracil in G:U mismatches. Has no lyase activity. Was first identified as methyl-CpG-binding protein.|
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Provided below are standard protocols that you may find useful for product applications.
MBD4 (methyl-CpG-binding domain protein 4) belongs to the MBD family of nuclear proteins that bind to methylated CpG sites and represses gene transcription. Human proteins MECP2, MBD1, MBD2, and MBD4, except MBD3 bind specifically to methylated DNA. MBD4 exhibits thymine glycosylase activity and is known to play an important function in DNA mismatch repair and apoptosis.
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