|Calculated MW||78459 Da|
|Positive Control||Jurkat cell lysate|
|Application & Usage||The antibody can be used in Western Blot analysis (0.5-4 µg/ml). However, the optimal concentrations should be determined individually. Blocking peptide is available separately.|
|Other Names||Protein arginine N-methyltransferase 7, PRMT7|
|Formulation||100 µg (0.5 mg/ml) affinity purified rabbit anti-PRMT7 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 5 mM EDTA and 0.01% thimerosal.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||PRMT7 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Arginine methyltransferase that can both catalyze the formation of omega-N monomethylarginine (MMA) and symmetrical dimethylarginine (sDMA), with a preference for the formation of MMA. Specifically mediates the symmetrical dimethylation of arginine residues in the small nuclear ribonucleoproteins Sm D1 (SNRPD1) and Sm D3 (SNRPD3); such methylation being required for the assembly and biogenesis of snRNP core particles. Specifically mediates the symmetric dimethylation of histone H4 'Arg-3' to form H4R3me2s. Plays a role in gene imprinting by being recruited by CTCFL at the H19 imprinted control region (ICR) and methylating histone H4 to form H4R3me2s, possibly leading to recruit DNA methyltransferases at these sites. May also play a role in embryonic stem cell (ESC) pluripotency. Also able to mediate the arginine methylation of histone H2A and myelin basic protein (MBP) in vitro; the relevance of such results is however unclear in vivo.|
|Cellular Location||Cytoplasm, cytosol. Nucleus|
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Provided below are standard protocols that you may find useful for product applications.
Arginine methylation is an important protein modification catalyzed by arginine methyltransferase such as PRMT7. PRMT7 has two methyltransferase domains each containing a putative AdoMet binding motif. Arginine methylation has been implicated in signal transduction RNA processing and splicing.
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