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Fas/Apo1 Antibody

Rabbit Polyclonal Antibody

  • WB - Fas/Apo1 Antibody ABV10051
    Western blot analysis of Fas expression with rat kidney tissue lysate.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P25446
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 37437 Da
Additional Information
Gene ID 14102
Application & Usage Western blotting (0.5-4 µg/ml) and Immunohistochemistry (5 µg/ml). However, the optimal conditions should be determined individually. The purified antibody detects a 55 kDa of glycosylated Fas, a 35 kDa native form of Fas and a 29 kDa of cleaved Fas fragment on SDS-PAGE immunoblots.
Other Names ALPS1A , APO-1, APT1, CD95 , FAS1, FAIM2, FASTM, LFG, NMP35
Target/Specificity Fas/Apo-1
Antibody Form Liquid
Appearance Colorless liquid
Formulation 100 µg (0.5 mg/ml) affinity purified polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.
Handling The antibody solution should be gently mixed before use.
Reconstitution & Storage -20 °C
Background Descriptions
PrecautionsFas/Apo1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name Fas
Synonyms Apt1, Tnfrsf6
Function Receptor for TNFSF6/FASLG. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death- inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. FAS- mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both (By similarity).
Cellular Location Membrane; Single-pass type I membrane protein
Tissue Location Detected in various tissues including thymus, liver, lung, heart, and adult ovary
Research Areas
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Fas/Apo-1/CD95 is a cell surface type-I membrane glycoprotein with an apparent molecular weight of 44 kDa on SDS-PAGE. Fas is a member of the TNFR family that includes TNFR1, TNFR2, CD27, CD30, and CD40. Binding of Fas-ligand (FasL) to Fas or crosslinking of Fas by anti-Fas monoclonal antibodies leads to apoptosis in Fas expressing cells. Both Fas and TNF-R contain the 70 amino acids cytoplasmic “death domain” that is responsible for transmitting signal for apoptosis.

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$ 245.00
Cat# ABV10051
(40 western blots)
Availability: 5-7days
Bulk Size
Seasonal Special on Bulk Order
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