Fas/Apo1 Antibody
Rabbit Polyclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, IHC |
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Primary Accession | P25446 |
Reactivity | Human, Mouse, Rat |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 37437 Da |
Gene ID | 14102 |
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Application & Usage | Western blotting (0.5-4 µg/ml) and Immunohistochemistry (5 µg/ml). However, the optimal conditions should be determined individually. The purified antibody detects a 55 kDa of glycosylated Fas, a 35 kDa native form of Fas and a 29 kDa of cleaved Fas fragment on SDS-PAGE immunoblots. |
Other Names | ALPS1A , APO-1, APT1, CD95 , FAS1, FAIM2, FASTM, LFG, NMP35 |
Target/Specificity | Fas/Apo-1 |
Antibody Form | Liquid |
Appearance | Colorless liquid |
Formulation | 100 µg (0.5 mg/ml) affinity purified polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal. |
Handling | The antibody solution should be gently mixed before use. |
Reconstitution & Storage | -20 °C |
Background Descriptions | |
Precautions | Fas/Apo1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | Fas |
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Synonyms | Apt1, Tnfrsf6 |
Function | Receptor for TNFSF6/FASLG. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen- stimulated suicide of mature T-cells, or both (By similarity). |
Cellular Location | Cell membrane {ECO:0000250|UniProtKB:P51867}; Single-pass type I membrane protein {ECO:0000250|UniProtKB:P51867} Membrane raft {ECO:0000250|UniProtKB:P25445} |
Tissue Location | Detected in various tissues including thymus, liver, lung, heart, and adult ovary. |
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Provided below are standard protocols that you may find useful for product applications.
Background
Fas/Apo-1/CD95 is a cell surface type-I membrane glycoprotein with an apparent molecular weight of 44 kDa on SDS-PAGE. Fas is a member of the TNFR family that includes TNFR1, TNFR2, CD27, CD30, and CD40. Binding of Fas-ligand (FasL) to Fas or crosslinking of Fas by anti-Fas monoclonal antibodies leads to apoptosis in Fas expressing cells. Both Fas and TNF-R contain the 70 amino acids cytoplasmic “death domain” that is responsible for transmitting signal for apoptosis.
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