|Application ||WB, IHC|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||37437 Da|
|Application & Usage||Western blotting (0.5-4 µg/ml) and Immunohistochemistry (5 µg/ml). However, the optimal conditions should be determined individually. The purified antibody detects a 55 kDa of glycosylated Fas, a 35 kDa native form of Fas and a 29 kDa of cleaved Fas fragment on SDS-PAGE immunoblots.|
|Other Names||ALPS1A , APO-1, APT1, CD95 , FAS1, FAIM2, FASTM, LFG, NMP35|
|Formulation||100 µg (0.5 mg/ml) affinity purified polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||Fas/Apo1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Receptor for TNFSF6/FASLG. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death- inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. FAS- mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both (By similarity).|
|Cellular Location||Membrane; Single-pass type I membrane protein|
|Tissue Location||Detected in various tissues including thymus, liver, lung, heart, and adult ovary|
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Provided below are standard protocols that you may find useful for product applications.
Fas/Apo-1/CD95 is a cell surface type-I membrane glycoprotein with an apparent molecular weight of 44 kDa on SDS-PAGE. Fas is a member of the TNFR family that includes TNFR1, TNFR2, CD27, CD30, and CD40. Binding of Fas-ligand (FasL) to Fas or crosslinking of Fas by anti-Fas monoclonal antibodies leads to apoptosis in Fas expressing cells. Both Fas and TNF-R contain the 70 amino acids cytoplasmic “death domain” that is responsible for transmitting signal for apoptosis.
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