|Application ||WB, IHC, FC, IP|
|Calculated MW||27716 Da|
|Positive Control||Human T-cells|
|Application & Usage||Western blot, Immunoprecipitation, Immunohistochemistry, flow cytometry. However, the optimal conditions should be determined individually.|
|Other Names||Granzyme B, 188.8.131.52, C11, CTLA-1, Cathepsin G-like 1, CTSGL1, Cytotoxic T-lymphocyte proteinase 2, Lymphocyte protease, Fragmentin-2, Granzyme-2, Human lymphocyte protein, HLP, SECT, T-cell serine protease 1-3E, GZMB, CGL1, CSPB, CTLA1, GRB|
|Formulation||100 µg (0.5 mg/ml) affinity purified mouse monoclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||Granzyme B Antibody (Clone B18.1) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||CGL1, CSPB, CTLA1, GRB|
|Function||This enzyme is necessary for target cell lysis in cell- mediated immune responses. It cleaves after Asp. Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -7, -9 and 10 to give rise to active enzymes mediating apoptosis.|
|Cellular Location||Cytoplasmic granule. Note=Cytoplasmic granules of cytolytic T-lymphocytes and natural killer cells|
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Provided below are standard protocols that you may find useful for product applications.
Granzyme B is a 27 kDa serine protease stored in granules of activated cytotoxic T cells and NK cells. Upon target cell contact, granzyme B is directionally exocytosed and assisted by perforin, enters the target cell. With its unique substrate specificity (granzyme B cleaves after Asp), granzyme B processes and activates various pro-caspases thereby inducing apoptosis in the target cell.
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