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TCF3/E2A Antibody

Rabbit Polyclonal Antibody

     
  • WB - TCF3/E2A Antibody ABV10067-30
    Western blot analysis of TCF3/E2A using Jurkat cell lysate (Lane 1 & 2)
  • SPECIFICATION
  • CITATIONS
  • PROTOCOLS
  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB
Primary Accession Q9JMG6
Other Accession D4ABE2
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Additional Information
Positive Control Jurkat cell lysate
Application & Usage Western Blot analysis (1 µg/ml). However, the optimal concentrations should be determined individually. Blocking peptide is available separately.
Other Names TCF3 fusion partner homolog, Protein amida, Tfpt, Amida
Target/Specificity TCF3/E2A
Antibody Form Liquid
Appearance Colorless liquid
Formulation 100 µg (0.5 mg/ml) affinity purified rabbit anti- TCF3/E2A polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 5 mM EDTA and 0.01% thimerosal.
Handling The antibody solution should be gently mixed before use.
Reconstitution & Storage -20 °C
Background Descriptions
PrecautionsTCF3/E2A Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name Tfpt
Synonyms Amida
Function Appears to promote apoptosis in a p53/TP53-independent manner.
Cellular Location Nucleus.
Tissue Location Ubiquitously expressed. Abundant in the brain. EMBL; AC103574; -; NOT_ANNOTATED_CDS; Genomic_DNA EMBL; AB029495; BAA90702.1; -; mRNA RefSeq; NP_620225.1; NM_138870.1 RefSeq; XP_006228098.1; XM_006228036.3 UniGene; Rn.12922; - BioGrid; 250095; 2 IntAct; Q9JMG6; 1 STRING; 10116.ENSRNOP00000018913; - iPTMnet; Q9JMG6; - PhosphoSitePlus; Q9JMG6; - PaxDb; Q9JMG6; - PRIDE; Q9JMG6; - GeneID; 85423; - KEGG; rno:85423; - UCSC; RGD:620839; rat CTD; 29844; - RGD; 620839; Tfpt eggNOG; ENOG410IXD0; Eukaryota eggNOG; ENOG4111UBX; LUCA HOGENOM; HOG000139148; - HOVERGEN; HBG056588; - InParanoid; Q9JMG6; - KO; K11670; - PhylomeDB; Q9JMG6; - TreeFam; TF338152; - PRO; PR:Q9JMG6; - Proteomes; UP000002494; Unplaced Bgee; ENSRNOG00000013980; - GO; GO:0005737; C:cytoplasm; IDA:UniProtKB GO; GO:0005634; C:nucleus; IDA:UniProtKB GO; GO:0003677; F:DNA binding; IDA:UniProtKB GO; GO:0046982; F:protein heterodimerization activity; IDA:RGD GO; GO:0019901; F:protein kinase binding; IPI:UniProtKB GO; GO:0006915; P:apoptotic process; IDA:UniProtKB GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-KW GO; GO:0006281; P:DNA repair; IEA:UniProtKB-KW GO; GO:0008584; P:male gonad development; IEP:RGD GO; GO:0043065; P:positive regulation of apoptotic process; IDA:RGD GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:UniProtKB-KW GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-KW InterPro; IPR033555; TFPT PANTHER; PTHR35084; PTHR35084; 1 1: Evidence at protein level; Apoptosis; Complete proteome; DNA damage; DNA recombination; DNA repair; Nucleus; Phosphoprotein; Reference proteome; Transcription; Transcription regulation CHAIN 1 259 TCF3 fusion partner homolog /FTId=PRO_0000254583 MOD_RES 167 167 Phosphoserine {ECO:0000250|UniProtKB:P0C1Z6} MOD_RES 172 172 Phosphothreonine {ECO:0000250|UniProtKB:P0C1Z6} MOD_RES 180 180 Phosphoserine MOD_RES 188 188 Phosphoserine {ECO:0000250|UniProtKB:P0C1Z6} MOD_RES 203 203 Phosphothreonine {ECO:0000250|UniProtKB:P0C1Z6} MOD_RES 255 255 Phosphoserine {ECO:0000250|UniProtKB:P0C1Z6} SEQUENCE 259 AA; 28908 MW; 674408B3F3FB6863 CRC64; MELEQREGTM AAVGFEEFSA PPGSELALPP LFGGHILESE LETEVEFVSG GLGDSGLRER DEEEEAARGR RRRQRELNRR KYQALGRRCR EIEQVNERVL NRLHQVQRIT RRLQQERRFL MRVLDSYGDD YRDSQFTIVL EDDGSQGTDV PTPGNVENEP PEKEGLSPPQ RTTATLDPSS PAPGEGPSGR KRRRAPRAAS SLTPELAPVQ VGAEGWGQGV IKVEEDFGFE ADEALDSSWV SRGPDKLLPY PTLASPPFD
Citations (0)

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Background

Heterodimers between TCF3 and tissue specific basic helix loop helix (bHLH) proteins play important roles in determining tissue specific cell fate during embryogenesis, like muscle or early B cell differentiation. Dimers bind DNA on E box motifs: 5' CANNTG 3'.Binds to the kappa E2 site in the kappa immunoglobulin gene enhancer.

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$ 89.00
$ 245.00
Cat# ABV10067-30
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