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>   home   >   Products   >   Primary Antibodies   >   Hsp70 Antibody (Clone S5)   

Hsp70 Antibody (Clone S5)

Mouse Monoclonal Antibody

Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P34932
Other Accession AAA02807
Reactivity Human, Mouse, Rat, Rabbit, Hamster, Monkey, Bovine
Host Mouse
Clonality Monoclonal
Isotype Mouse IgG1
Clone Names S5
Calculated MW 94331 Da
Additional Information
Gene ID 3308
Application & Usage Western blot analysis (1 µg/ml), immunoprecipitation (5-10 µg/ml), Immunohistochemistry (5-20 µg/ml, frozen & paraffin), and FACS analysis. However, the optimal conditions should be determined individually. The antibody recognizes a 70 kDa protein, corresponding to the apparent molecular weight of the inducible Hsp70 on SDS-PAGE immunoblots. This antibody has no reactivity with the constitutive Hsc70 (Hsp73).
Other Names Heat shock 70 kDa protein 4, HSP70RY, Heat shock 70-related protein APG-2, HSPA4, APG2
Target/Specificity Hsp70
Antibody Form Liquid
Appearance Colorless liquid
Formulation 100 µg (0.5 mg/ml) in PBS containing 1 mg/ml BSA and 1.5 mM sodium azide and 50% glycerol.
Handling The antibody solution should be gently mixed before use.
Reconstitution & Storage -20 °C
Background Descriptions
PrecautionsHsp70 Antibody (Clone S5) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name HSPA4
Synonyms APG2
Cellular Location Cytoplasm.
Citations (0)

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Hsp70, one class of stress proteins, is comprised of multiple members, all of which bind ATP in vitro, but are localized within different intracellular compartments. These include: a) the constitutive Hsc70 (or cognate) present within the cytosol/nucleus; b) the highly stress-inducible Hsp70 present within the cytosol/ nucleus/nucleolus; c) the constitutive glucose regulated 78 kDa (or BiP) protein present within the lumen of the endoplasmic reticulum; and d) the glucose regulated 75 kDa protein present within the mitochondria. Hsp70 is typically not expressed in the cell under normal growth conditions, but is expressed at high levels in the cell experiencing stress. Consequently, detection of Hsp70 using an antibody specific for the inducible form is quite useful in ascertaining whether a stress response has occurred in the cell.

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$ 295.00
Cat# ABV10073
(40 western blots)
Availability: 5-7days
Bulk Size
Seasonal Special on Bulk Order
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