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GLP-1 Antibody (Clone HGL-B5)

Mouse Monoclonal Antibody

  • E - GLP-1 Antibody (Clone HGL-B5) ABV10081
    Anti-human GLP1 antibody was used at 1 μg per well for detection of antigen (peptide, 1 μg serially diluted and coated). The antibody at 10μg/ml concentration can sensitively detect coated antigen up to 2 ng per well.
  • IF - GLP-1 Antibody (Clone HGL-B5) ABV10081
    Antigen retrieval was done with Tris-EDTA, pH9.0 in pressure cooker for 20 minutes. Optimum staining of L-type cells secreting GLP1 was observed.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P01275
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype Mouse IgG1
Clone Names HGL-B5
Calculated MW 20909 Da
Additional Information
Gene ID 2641
Application & Usage The antibody can be used for indirect ELISA and IF.
Other Names Glucagon, Glicentin, Glicentin-related polypeptide, GRPP, Oxyntomodulin, OXM, OXY, Glucagon-like peptide 1, GLP-1, Glucagon-like peptide 1(7-37), GLP-1(7-37), Glucagon-like peptide 1(7-36), GLP-1(7-36), Glucagon-like peptide 2, GLP-2, GCG
Target/Specificity GLP-1
Antibody Form Liquid
Appearance Colorless liquid
Formulation 100 µg (1.0 mg/ml) purified mouse monoclonal antibody supplied in PBS with 0.05% (W/V) sodium azide.
Handling The antibody solution should be gently mixed before use.
Reconstitution & Storage -20 °C
Background Descriptions
PrecautionsGLP-1 Antibody (Clone HGL-B5) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name GCG
Function Glucagon plays a key role in glucose metabolism and homeostasis. Regulates blood glucose by increasing gluconeogenesis and decreasing glycolysis. A counterregulatory hormone of insulin, raises plasma glucose levels in response to insulin-induced hypoglycemia. Plays an important role in initiating and maintaining hyperglycemic conditions in diabetes. GLP-2 stimulates intestinal growth and up-regulates villus height in the small intestine, concomitant with increased crypt cell proliferation and decreased enterocyte apoptosis. The gastrointestinal tract, from the stomach to the colon is the principal target for GLP-2 action. Plays a key role in nutrient homeostasis, enhancing nutrient assimilation through enhanced gastrointestinal function, as well as increasing nutrient disposal. Stimulates intestinal glucose transport and decreases mucosal permeability. Glicentin may modulate gastric acid secretion and the gastro-pyloro-duodenal activity. May play an important role in intestinal mucosal growth in the early period of life.
Cellular Location Secreted.
Tissue Location Glucagon is secreted in the A cells of the islets of Langerhans. GLP-1, GLP-2, oxyntomodulin and glicentin are secreted from enteroendocrine cells throughout the gastrointestinal tract. GLP-1 and GLP-2 are also secreted in selected neurons in the brain
Research Areas
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Glucagon-like peptide-1 (GLP-1) is identified as a gut hormone with potent insulin-releasing activity. GLP-1 possesses several physiological properties that make it a subject of intensive investigation as a potential treatment of diabetes mellitus. In addition, GLP-1 has other biological functions such as reduction of food intake and deceleration of gastric emptying.

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$ 265.00
Cat# ABV10081
(40 western blots)
Availability: 5-7days
Bulk Size
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