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GLP-1 Antibody (Clone HGL-B5)

Mouse Monoclonal Antibody

     
  • E - GLP-1 Antibody (Clone HGL-B5) ABV10081-100
    Anti-human GLP1 antibody was used at 1 μg per well for detection of antigen (peptide, 1 μg serially diluted and coated). The antibody at 10μg/ml concentration can sensitively detect coated antigen up to 2 ng per well.
    detail
  • IF - GLP-1 Antibody (Clone HGL-B5) ABV10081-100
    Antigen retrieval was done with Tris-EDTA, pH9.0 in pressure cooker for 20 minutes. Optimum staining of L-type cells secreting GLP1 was observed.
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IF, E
Primary Accession P01275
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype Mouse IgG1
Clone Names HGL-B5
Calculated MW 20909 Da
Additional Information
Gene ID 2641
Application & Usage The antibody can be used for indirect ELISA and IF.
Other Names Glucagon, Glicentin, Glicentin-related polypeptide, GRPP, Oxyntomodulin, OXM, OXY, Glucagon-like peptide 1, GLP-1, Glucagon-like peptide 1(7-37), GLP-1(7-37), Glucagon-like peptide 1(7-36), GLP-1(7-36), Glucagon-like peptide 2, GLP-2, GCG
Target/Specificity GLP-1
Antibody Form Liquid
Appearance Colorless liquid
Formulation 100 µg (1.0 mg/ml) purified mouse monoclonal antibody supplied in PBS with 0.05% (W/V) sodium azide.
Handling The antibody solution should be gently mixed before use.
Reconstitution & Storage -20 °C
Background Descriptions
PrecautionsGLP-1 Antibody (Clone HGL-B5) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name GCG (HGNC:4191)
Function [Glucagon]: Plays a key role in glucose metabolism and homeostasis. Regulates blood glucose by increasing gluconeogenesis and decreasing glycolysis. A counterregulatory hormone of insulin, raises plasma glucose levels in response to insulin-induced hypoglycemia. Plays an important role in initiating and maintaining hyperglycemic conditions in diabetes.
Cellular Location Secreted.
Tissue Location [Glucagon]: Secreted in the A cells of the islets of Langerhans. [Glucagon-like peptide 2]: Secreted from enteroendocrine cells throughout the gastrointestinal tract. Also secreted in selected neurons in the brain [Oxyntomodulin]: Secreted from enteroendocrine cells throughout the gastrointestinal tract
Research Areas
Citations (0)
citation

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Background

Glucagon-like peptide-1 (GLP-1) is identified as a gut hormone with potent insulin-releasing activity. GLP-1 possesses several physiological properties that make it a subject of intensive investigation as a potential treatment of diabetes mellitus. In addition, GLP-1 has other biological functions such as reduction of food intake and deceleration of gastric emptying.

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Discontinued
Cat# ABV10081-100
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