|Application ||WB, IHC, E|
|Reactivity||Human, Pig, Bovine|
|Calculated MW||11981 Da|
|Application & Usage||The antibody can be used for indirect ELISA, WB and IHC.|
|Formulation||100 µg (1.0 mg/ml) purified mouse monoclonal antibody supplied in PBS with 0.05% (W/V) sodium azide.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||Proinsulin Antibody (Clone HPI-B5) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.|
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Provided below are standard protocols that you may find useful for product applications.
Proinsulin is a prohormone precursor to Insulin, made by the pancreas in response to increased blood s µgar. Proinsulin is 39 amino acids longer than the mature Insulin, of which 4 are completely lost and the remaining 35 forms the C-peptide. The measurement of Proinsulin in serum provides a useful, valuable information for the diagnosis of insulinomas. Proinsulin levels have also been shown to be elevated in non-insulin dependent diabetics (NIDDM), in insulin dependent diabetics (IDDM) and other clinical situations. The antibody recognizes insulin and recombinant proinsulin, but does not react with synthetic C-peptide.
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