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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   Proinsulin Antibody (Clone HPI-B5)   

Proinsulin Antibody (Clone HPI-B5)

Mouse Monoclonal Antibody

     
  • E - Proinsulin Antibody (Clone HPI-B5) ABV10083
    Serial dilution of the recombinant insulin was done to check the affinity. The antibody used at 10 μg/ml can sensitively detect antigen up to 4 ng per well.
  • IHC - Proinsulin Antibody (Clone HPI-B5) ABV10083
    Antigen retrieval was done with Tris-EDTA, pH 9.0 in pressure cooker for 20 minutes. Optimum staining of the islet cell was observed
  • SPECIFICATION
  • CITATIONS
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC, E
Primary Accession P01308
Reactivity Human, Pig, Bovine
Host Mouse
Clonality Monoclonal
Isotype Mouse IgG1
Clone Names HPI-B5
Calculated MW 11981 Da
Additional Information
Gene ID 3630
Application & Usage The antibody can be used for indirect ELISA, WB and IHC.
Other Names Proinsulin
Target/Specificity ProInsulin
Antibody Form Liquid
Appearance Colorless liquid
Formulation 100 µg (1.0 mg/ml) purified mouse monoclonal antibody supplied in PBS with 0.05% (W/V) sodium azide.
Handling The antibody solution should be gently mixed before use.
Reconstitution & Storage -20 °C
Background Descriptions
PrecautionsProinsulin Antibody (Clone HPI-B5) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name INS
Function Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.
Cellular Location Secreted.
Research Areas
Citations (0)

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Background

Proinsulin is a prohormone precursor to Insulin, made by the pancreas in response to increased blood s µgar. Proinsulin is 39 amino acids longer than the mature Insulin, of which 4 are completely lost and the remaining 35 forms the C-peptide. The measurement of Proinsulin in serum provides a useful, valuable information for the diagnosis of insulinomas. Proinsulin levels have also been shown to be elevated in non-insulin dependent diabetics (NIDDM), in insulin dependent diabetics (IDDM) and other clinical situations. The antibody recognizes insulin and recombinant proinsulin, but does not react with synthetic C-peptide.

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$ 265.00
Cat# ABV10083
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