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>   home   >   Products   >   Primary Antibodies   >   PYY3-36 Antibody (Clone RPY-B12)   

PYY3-36 Antibody (Clone RPY-B12)

Mouse Monoclonal Antibody

  • E - PYY3-36 Antibody (Clone RPY-B12) ABV10084-100
    Serial dilution of the antibody starting with 5 μg/well was done to check for the affinity. 0.5 μg of coated antigen can be sensitively detected by anti-rat PYY3-36 antibody used at 3 ng/well.
  • IF - PYY3-36 Antibody (Clone RPY-B12) ABV10084-100
    Antigen retrieval was done with Tris-EDTA, pH 9.0 in pressure cooker. Endocrine cells from the mucosal layer of colon labeled by the antibody display optimum cytoplasmic staining.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P10631
Reactivity Rat
Host Mouse
Clonality Monoclonal
Isotype Mouse IgG1
Clone Names RPY-B12
Calculated MW 11121 Da
Additional Information
Gene ID 287730
Positive Control Rat Colon Tissue
Application & Usage The antibody can be used for indirect ELISA for detection of antigen (recommended use at 1-2 µg/ml) and Immunofluorescence (recommended use at 20-40 µg/ml).
Other Names PYY3-36, Peptide YY
Target/Specificity PYY3-36
Antibody Form Liquid
Appearance Colorless liquid
Formulation 100 µg (0.5 mg/ml) affinity purified mouse monoclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 0.5% BSA, and 0.01% thimerosal.
Handling The antibody solution should be gently mixed before use.
Reconstitution & Storage -20 °C
Background Descriptions
PrecautionsPYY3-36 Antibody (Clone RPY-B12) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name Pyy
Function This gut peptide inhibits exocrine pancreatic secretion, has a vasoconstrictory action and inhibitis jejunal and colonic mobility.
Cellular Location Secreted.
Citations (0)

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Peptide YY (PYY), a member of a neuropeptide Y (NPY family) is a 36 AA peptide secreted from the L cells of the gastrointestinal tract in response to food intake. The release of PYY is stimulated by nutrients within the lower small intestine and the colon, which in turn decreases food intake by inhibiting gut motility. It acts as an “ileal brake” to cause a sense of satiety. PYY3-36 is produced by the dipeptidyl peptidase-IV digestion of polypeptide YY (PYY).

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$ 265.00
Cat# ABV10084-100
Availability: 5-7days
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