|Application ||WB, IHC|
|Reactivity||Human, Mouse, Rat, Rabbit, Hamster, Monkey, Bovine|
|Calculated MW||50130 Da|
|Application & Usage||Western blotting (0.5-4 µg/ml) and in Immunohistochemistry (10-20 µg/ml). However, the optimal conditions should be determined individually. The antibody detects cdc42 of human, mouse, rat, and bovine origins. |
|Other Names||TNF-R1, Tumor Necrosis Factor type I, TNFRSF1A, TNFAR, TNF-R55, TNFR60, p55, CD120a|
|Formulation||100 µg (0.2 mg/ml) affinity purified rabbit anti-TNF-R1 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||TNF-R1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Receptor for TNFSF2/TNF-alpha and homotrimeric TNFSF1/lymphotoxin-alpha. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate- specific cysteine proteases) mediating apoptosis (By similarity).|
|Cellular Location||Cell membrane; Single-pass type I membrane protein. Golgi apparatus membrane; Single-pass type I membrane protein|
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Provided below are standard protocols that you may find useful for product applications.
Tumor necrosis factor receptor 1 and 2 (TNF-R1 and TNF-R2) are 55 and 75 kDa. While TNF-R1 and TNF-R2 share 28% sequence homology in the extracellular domains, their intracellular domains lack sequence homology, s µggesting that they differ in their internal signal transduction pathways. TNF-R1 contains an approximately 80 amino acid death domain near its carboxy terminus capable of transmitting an apoptotic signal thro µgh its interaction with TRADD (TNF-R1 associated death domain protein), and subsequent interactions with FADD. TNF-R1 can also activate the transcription factor NFkB via TRAF2 (TNF receptor associated factor 2). The cytoplasmic domain of TNF-R1 can directly interact with Jak kinase, thereby activating the JAK/STAT signal transduction cascade.
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