|Reactivity||Human, Mouse, Rat|
|Positive Control||Jurkat cell lysate|
|Application & Usage||Western blotting (2-4 µg/ml). However, the optimal conditions should be determined individually.|
|Other Names||Lysine-specific histone demethylase 1A (BRAF35-HDAC complex protein BHC110) (Flavin-containing amine oxidase domain-containing protein 2)|
|Formulation||100 µg (0.5 mg/ml) affinity purified rabbit anti- LSD1/AOF2 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 5 mM EDTA and 0.01% thimerosal.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||LSD1/AOF2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
Lysine-specific demethylase 1(LSD1) is also known as AOF2, is a nuclear amine oxidase. LSD1 contain a Swirm domain, a FAD-binding motif and an amine oxidase domain that can act as a histone demethylase as well as transcription cofactor. LSD1 activity is inhibited by the amine oxidase inhibitors pargyline, deprenyl, clorgyline and tranylcypromine
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