|Reactivity||Human, Mouse, Rat|
|Calculated MW||34277 Da|
|Application & Usage||Western blot analysis (0.5-4 µg/ml). However, the optimal conditions should be determined individually. The antibody detects the full length (35-37 kDa) and the large fragment (20-25 kDa) of processed caspase-7 by Western blotting. Mouse small intestine tissue lysate can be used as a positive control.|
|Other Names||CASP7, CASP-7, ICE-LAP3, CMH-1, MCH3, EC 188.8.131.52|
|Formulation||100 µg (200 µg/ml) affinity purified rabbit anti-caspase-7 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||Caspase-7 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Involved in the activation cascade of caspases responsible for apoptosis execution. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly- 217' bond. Overexpression promotes programmed cell death.|
|Tissue Location||Highly expressed in lung, skeletal muscle, liver, kidney, spleen and heart, and moderately in testis. No expression in the brain|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
firstname.lastname@example.org, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
Caspase-7 is a 34 kDa protein consisting of two subunits and a short prodomain. The procaspase-7 can be activated by cleavage by upstream caspases to form active caspase-7 in apoptosis. Once activated, caspase-7 cleaves many of the same substrates as caspase-3, including poly (ADP-ribose) polymerase (PARP) and sterol regulatory element binding proteins (SREBPs).
If you have used an Abgent product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at email@example.com.