Caspase-9 Antibody
Rabbit Polyclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB |
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Primary Accession | Q8C3Q9 |
Other Accession | BAA86895 |
Reactivity | Human, Mouse, Rat |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 49979 Da |
Gene ID | 12371 |
---|---|
Application & Usage | Western blotting (0.5-4 µg/ml). However, the optimal conditions should be determined individually. The antibody detects proform (~46 kDa), intermediate (~33-36 kDa), and the fully cleaved (~20-25 kDa) caspase-9. |
Other Names | CASP-9 , EC 3.4.22.62 , ICE-like apoptotic protease 6 , ICE-LAP6, Apoptotic protease Mch-6 , Apoptotic protease-activating factor 3 , Apaf-3 |
Target/Specificity | Caspase-9 |
Antibody Form | Liquid |
Appearance | Colorless liquid |
Formulation | 100 µg (0.2 mg/ml) affinity purified rabbit anti-caspase-9 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal. |
Handling | The antibody solution should be gently mixed before use. |
Reconstitution & Storage | -20 °C |
Background Descriptions | |
Precautions | Caspase-9 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | Casp9 |
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Synonyms | Mch6 |
Function | Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates effector caspases caspase-3 (CASP3) or caspase-7 (CASP7). Promotes DNA damage- induced apoptosis in a ABL1/c-Abl-dependent manner. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP). |
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Provided below are standard protocols that you may find useful for product applications.
Background
Caspase-9 is one of the most important caspases among the caspase family members. Caspase-9 is synthesized as inactive pro-enzyme that is processed in cells undergoing apoptosis. The processed caspase-9 consists of large (32-35 kD) and small (10 kD) subunits which associate to form an active enzyme. Activated caspase-9 in turn cleaves and activates caspase-3, one of the proteases responsible for the proteolytic cleavage of many key proteins in apoptosis.
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