|Reactivity||Human, Mouse, Rat|
|Calculated MW||46555 Da|
|Positive Control||Rat kidney tissue lysate|
|Application & Usage||Western blot analysis (1-4 µg/ml). However, the optimal conditions should be determined individually. Blocking peptide is available separately.|
|Other Names||Medium-chain specific acyl-CoA dehydrogenase|
|Formulation||100 µg (0.5 mg/ml) affinity purified rabbit anti-ACADM polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 5 mM EDTA and 0.01% thimerosal.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||ACADM Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Acyl-CoA dehydrogenase specific for acyl chain lengths of 4 to 16 that catalyzes the initial step of fatty acid beta- oxidation. Utilizes the electron transfer flavoprotein (ETF) as an electron acceptor to transfer electrons to the main mitochondrial respiratory chain via ETF-ubiquinone oxidoreductase (ETF dehydrogenase).|
|Cellular Location||Mitochondrion matrix.|
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Provided below are standard protocols that you may find useful for product applications.
Medium-chain acyl-CoA dehydrogenase (MCAD, ACADM) is a homotetramer enzyme that catalyzes the initial step of the mitochondrial fatty acid beta-oxidation pathway.
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