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HK2 (Hexokinase II) (NT) Antibody
Rabbit Polyclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC, E |
|---|---|
| Primary Accession | P52789 |
| Reactivity | Human |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 102380 Da |
| Gene ID | 3099 |
|---|---|
| Positive Control | Western Blot: A375 cell lysate Immunohistochemistry: Human skeletal muscle tissue |
| Application & Usage | The antibody can be used for ELISA (0.25 µg/ml), Western blotting (0.5 – 2.5 µg/ml) and Immunohistochemistry (2.5 – 5.0 µg/ml). |
| Other Names | Hexokinase type II, HK II, Muscle form hexokinase |
| Target/Specificity | HK2 (Hexokinase II) |
| Antibody Form | Liquid |
| Appearance | Colorless liquid |
| Formulation | 100 µg (0.25 mg/ml) purified rabbit Ig polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. |
| Handling | The antibody solution should be gently mixed before use. |
| Reconstitution & Storage | -20 °C |
| Background Descriptions | |
| Precautions | HK2 (Hexokinase II) (NT) Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | HK2 (HGNC:4923) |
|---|---|
| Function | Catalyzes the phosphorylation of hexose, such as D-glucose and D-fructose, to hexose 6-phosphate (D-glucose 6-phosphate and D- fructose 6-phosphate, respectively) (PubMed:23185017, PubMed:26985301, PubMed:29298880). Mediates the initial step of glycolysis by catalyzing phosphorylation of D-glucose to D-glucose 6-phosphate (PubMed:29298880). Plays a key role in maintaining the integrity of the outer mitochondrial membrane by preventing the release of apoptogenic molecules from the intermembrane space and subsequent apoptosis (PubMed:18350175). |
| Cellular Location | Mitochondrion outer membrane; Peripheral membrane protein. Cytoplasm, cytosol Note=The mitochondrial-binding peptide (MBP) region promotes association with the mitochondrial outer membrane (PubMed:29298880) The interaction with the mitochondrial outer membrane via the mitochondrial-binding peptide (MBP) region promotes higher stability of the protein (PubMed:29298880). Release from the mitochondrial outer membrane into the cytosol induces permeability transition pore (PTP) opening and apoptosis (PubMed:18350175). |
| Tissue Location | Predominant hexokinase isozyme expressed in insulin-responsive tissues such as skeletal muscle |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
In vertebrates there are four major glucose-phosphorylating isoenzymes, designated hexokinase I, II, III, and IV. Hexokinase is an allosteric enzyme inhibited by its product GLC-6-P. Hexokinase activity is involved in the first step in several metabolic pathways. HK3 is bound to the outer mitochondrial membrane. Its hydrophobic N-terminal sequence may be involved in membrane bindng. It is the predominant hexokinase isozyme expressed in insuline-responsive tissues such as skeletal muscle. The N- and C-terminal halves of this hexokinase show extensive sequence similarity to each other. The catalytic activity is associated with the C-terminus while regulatory function is associated wiht the N-terminus. Altho µgh found in NIDDM patients, genetic variations of HK2 do not contribute to the disease.
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