Caspase-9 (Active) Antibody
Rabbit Polyclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, IP |
---|---|
Primary Accession | Q8C3Q9 |
Other Accession | BAA86895 |
Reactivity | Human |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 49979 Da |
Gene ID | 12371 |
---|---|
Application & Usage | Western blotting (0.5-4 µg/ml) and immunoprecipitation (10-20 µg/ml). However, the optimal concentrations should be determined individually. The anti-active caspase-9 antibody recognizes only the cleaved caspase-9 (37 kDa). It does not recognize full-length caspase-9 or any other caspases. |
Other Names | CASP-9, EC 3.4.22.62, ICE-like apoptotic protease 6, ICE-LAP6, Apoptotic protease Mch-6, Apoptotic protease-activating factor 3, Apaf-3 |
Target/Specificity | Caspase-9 (Active) |
Antibody Form | Liquid |
Appearance | Colorless liquid |
Formulation | 100 µg (0.2 mg/ml) affinity purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 50% glycerol, 0.5% BSA, and 0.02% thimerosal. |
Handling | The antibody solution should be gently mixed before use. |
Reconstitution & Storage | -20 °C |
Background Descriptions | |
Precautions | Caspase-9 (Active) Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | Casp9 |
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Synonyms | Mch6 |
Function | Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates effector caspases caspase-3 (CASP3) or caspase-7 (CASP7). Promotes DNA damage- induced apoptosis in a ABL1/c-Abl-dependent manner. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP). |
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Provided below are standard protocols that you may find useful for product applications.
Background
Caspases are synthesized as inactive pro-enzymes that are processed to active form in cells undergoing apoptosis. Caspase-9 is an important member of the caspase family. Upon induction of apoptosis, Cytochrome c released from mitochondria associates with pro-caspase-9 (47 kDa) and Apaf-1. The complex processes pro-caspase-9 into a large subunit (37 kDa/17 kDa) and a small subunit (10 kDa). Cleaved caspase-9 further processes other caspases including caspase-3 and caspase-6, to initiate a caspase cascade leading to apoptosis. The affinity purified antibody recognizing the active forms of caspase-9 provides a new tool for identifying apoptotic cell populations in both tissue sections and cultured cells.
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