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ACADSB Antibody

Rabbit Polyclonal Antibody

     
  • WB - ACADSB Antibody ABV10121-30
    Western blot analysis of ACADSB with mouse 3T3 cell lysate (Lane 1) and rat kidney tissue lysate (Lane 2).
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB
Primary Accession Q9DBL1
Reactivity Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 47874 Da
Additional Information
Gene ID 66885
Positive Control Mouse 3T3 cell lysate and rat kidney tissue lysate
Application & Usage The antibody can be used for Western blot analysis (1-4 µg/ml). However, the optimal conditions should be determined individually. Blocking peptide is available separately.
Other Names Short/branched chain specific acyl-CoA dehydrogenase, SBCAD
Target/Specificity ACADSB
Antibody Form Liquid
Appearance Colorless liquid
Formulation 100 µg (0.5 mg/ml) affinity purified rabbit anti-ACADSB polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 5 mM EDTA and 0.01% thimerosal.
Handling The antibody solution should be gently mixed before use.
Reconstitution & Storage -20 °C
Background Descriptions
PrecautionsACADSB Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name Acadsb {ECO:0000312|MGI:MGI:1914135}
Function Short and branched chain specific acyl-CoA dehydrogenase that catalyzes the removal of one hydrogen from C-2 and C-3 of the fatty acyl-CoA thioester, resulting in the formation of trans-2-enoyl-CoA. Among the different mitochondrial acyl-CoA dehydrogenases, acts specifically on short and branched chain acyl-CoA derivatives such as (S)-2-methylbutyryl-CoA as well as short straight chain acyl-CoAs such as butyryl-CoA (By similarity). Plays an important role in the metabolism of L-isoleucine by catalyzing the dehydrogenation of 2- methylbutyryl-CoA, one of the steps of the L-isoleucine catabolic pathway (By similarity). Can also act on valproyl-CoA, a metabolite of the valproic acid drug (By similarity).
Cellular Location Mitochondrion matrix {ECO:0000250|UniProtKB:P45954}
Research Areas
Citations (0)
citation

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Background

Short/branched chain acyl-CoA dehydrogenase(ACADSB) belongs to the acyl-CoA dehydrogenase family of enzymes that catalyze the dehydrogenation of acyl-CoA derivatives in the metabolism of fatty acids or branch chained amino acids. ACADSB catalyzes the degradation of L-isoleucine and has the greatest affinity towards (S)-2-methylbutyryl-CoA, isobutyryl-CoA and 2-methylhexanoyl-CoA as substrates.

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Discontinued
Cat# ABV10121-30
Size:

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