Holocytochrome c Antibody
Mouse Monoclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB |
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Primary Accession | P53701 |
Reactivity | Human, Mouse, Rat |
Host | Mouse |
Clonality | Monoclonal |
Isotype | Mouse IgG1 |
Calculated MW | 30602 Da |
Gene ID | 3052 |
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Application & Usage | Western blot analysis (5-10 µg/ml) and immunoprecipitation. The antibody detects a 12.0 kDa protein corresponding to the apparent molecular weight of cytochrome c. |
Other Names | Cytochrome c-type heme lyase, CCHL, 4.4.1.17, Holocytochrome c-type synthase, HCCS, CCHL |
Target/Specificity | Holocytochrome c |
Antibody Form | Liquid |
Appearance | Colorless liquid |
Formulation | 100 µg (0.2 mg/ml) purified IgG in PBS containing 50% glycerol, 1% BSA and 0.02% sodium azide. |
Handling | The antibody solution should be gently mixed before use. |
Reconstitution & Storage | -20 °C |
Background Descriptions | |
Precautions | Holocytochrome c Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | HCCS (HGNC:4837) |
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Synonyms | CCHL |
Function | Lyase that catalyzes the covalent linking of the heme group to the cytochrome C apoprotein to produce the mature functional cytochrome. |
Cellular Location | Mitochondrion inner membrane. Membrane; Lipid-anchor |
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Provided below are standard protocols that you may find useful for product applications.
Background
The precursor of cytochrome c, apocytochrome c, is synthesized in the cytoplasm. Upon translocation into mitochondria, cytochrome c acquires a heme moiety required for functionality in the mitochondrial respiration chain. The heme-bound form of cytochrome c is called holocytochrome c. When released into the cytosol, cytochrome c can activate caspases responsible for apoptosis.
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