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Calpain 1 Antibody
Rabbit Polyclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB |
|---|---|
| Primary Accession | P97571 |
| Reactivity | Human, Mouse, Rat, Rabbit, Pig, Bovine |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 82119 Da |
| Gene ID | 29153 |
|---|---|
| Application & Usage | Western blotting (0.5-4 µg/ml). However, the optimal concentrations should be determined individually. The anti-calpain I antibody recognizes the large subunit (80 kDa) of human Calpain 1. It does not recognize the smaller subunit of human Calpain 1. |
| Other Names | EC 3.4.22.52, CAPN2 |
| Target/Specificity | Calpain 1 |
| Antibody Form | Liquid |
| Appearance | Colorless liquid |
| Formulation | 100 µg (0.5 mg/ml) affinity purified rabbit anti-calpain 1 (large subunit) antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 0.5% BSA, and 0.01% thimerosal. |
| Handling | The antibody solution should be gently mixed before use. |
| Reconstitution & Storage | -20 °C |
| Background Descriptions | |
| Precautions | Calpain 1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | Capn1 {ECO:0000312|RGD:2267} |
|---|---|
| Function | Calcium-regulated non-lysosomal thiol-protease which catalyzes limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. Proteolytically cleaves CTBP1 at 'Asn-364', 'Gly-377' and 'His-399'. Cleaves and activates caspase-7 (CASP7). |
| Cellular Location | Cytoplasm {ECO:0000250|UniProtKB:P07384}. Cell membrane {ECO:0000250|UniProtKB:P07384}. Note=Translocates to the plasma membrane upon Ca(2+) binding. {ECO:0000250|UniProtKB:P07384} |
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Provided below are standard protocols that you may find useful for product applications.
Background
Calpains are cytosolic, calcium-dependent, cysteine proteases that are ubiquitously expressed in mammalian and avian tissues. Type I calpain requires micromolar amounts of calcium, while type II calpain requires millimolar amounts of calcium.
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