|Reactivity||Human, Mouse, Rat, Rabbit, Pig, Bovine|
|Calculated MW||82119 Da|
|Application & Usage||Western blotting (0.5-4 µg/ml). However, the optimal concentrations should be determined individually. The anti-calpain I antibody recognizes the large subunit (80 kDa) of human Calpain 1. It does not recognize the smaller subunit of human Calpain 1.|
|Other Names||EC 220.127.116.11, CAPN2|
|Formulation||100 µg (0.5 mg/ml) affinity purified rabbit anti-calpain 1 (large subunit) antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 0.5% BSA, and 0.01% thimerosal.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||Calpain 1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction.|
|Cellular Location||Cytoplasm. Cell membrane. Note=Translocates to the plasma membrane upon Ca(2+) binding.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
firstname.lastname@example.org, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
Calpains are cytosolic, calcium-dependent, cysteine proteases that are ubiquitously expressed in mammalian and avian tissues. Type I calpain requires micromolar amounts of calcium, while type II calpain requires millimolar amounts of calcium.
If you have used an Abgent product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at email@example.com.