Bcl-2 Antibody (Clone Bcl-2/100)
Mouse Monoclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, IHC, IP |
---|---|
Primary Accession | P10415 |
Other Accession | M13994 |
Reactivity | Human |
Host | Mouse |
Clonality | Monoclonal |
Isotype | Mouse IgG1 |
Clone Names | Bcl-2/100 |
Calculated MW | 26266 Da |
Gene ID | 596 |
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Application & Usage | Western blot (1-4 µg/ml), Immunoprecipitation, Immunohistochemistry, and Flow cytometry. However the optimal conditions should be determined individually. Recognize the 26 kDa human Bcl-2 |
Other Names | BCL2, BCL 2 |
Target/Specificity | Bcl-2 |
Antibody Form | Liquid |
Appearance | Colorless liquid |
Formulation | 100 µg (0.5 mg/ml) protein A purified from (low FBS containing) tissue culture supernatant. Purity was >95%. The antibody was formulated in PBS containing 1 mg/ml BSA and 1.5 mM sodium azide and 50% glycerol |
Handling | The antibody solution should be gently mixed before use. |
Reconstitution & Storage | -20 °C |
Background Descriptions | |
Precautions | Bcl-2 Antibody (Clone Bcl-2/100) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | BCL2 |
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Function | Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells (PubMed:1508712, PubMed:8183370). Regulates cell death by controlling the mitochondrial membrane permeability (PubMed:11368354). Appears to function in a feedback loop system with caspases (PubMed:11368354). Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1) (PubMed:11368354). Also acts as an inhibitor of autophagy: interacts with BECN1 and AMBRA1 during non-starvation conditions and inhibits their autophagy function (PubMed:18570871, PubMed:21358617, PubMed:20889974). May attenuate inflammation by impairing NLRP1- inflammasome activation, hence CASP1 activation and IL1B release (PubMed:17418785). |
Cellular Location | Mitochondrion outer membrane; Single-pass membrane protein. Nucleus membrane; Single-pass membrane protein. Endoplasmic reticulum membrane; Single-pass membrane protein. Cytoplasm {ECO:0000250|UniProtKB:P10417} |
Tissue Location | Expressed in a variety of tissues. |
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Provided below are standard protocols that you may find useful for product applications.
Background
Bcl-2 (B-cell leukemia/lymphoma 2) gene was discovered by walking the chromosomal breakpoints t(14;18). This gene consists of two exons and several transcripts that encode two different protein products, Bcl-2α and Bcl-2β. Both proteins are approximately 26-30 kDa and differ at their C-terminal region. Bcl-2 suppresses apoptosis (programmed cell death) and this property appears to be modulated by homodimerization or association with Bax and Bad, two cell death promoters
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