|Reactivity||Human, Mouse, Rat|
|Calculated MW||66901 Da|
|Application & Usage||Western blotting (0.5-4 µg/ml). A ~67 kDa band should be detected. Jurkat cell lysate can be used as a positive control. However, the optimal conditions for your testing samples should be determined individually.|
|Other Names||AIFM1, PDCD8, MGC111425|
|Formulation||100 µg (0.5 mg/ml) affinity purified rabbit anti-AIF polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||AIF Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Functions both as NADH oxidoreductase and as regulator of apoptosis. In response to apoptotic stimuli, it is released from the mitochondrion intermembrane space into the cytosol and to the nucleus, where it functions as a proapoptotic factor in a caspase-independent pathway. In contrast, functions as an antiapoptotic factor in normal mitochondria via its NADH oxidoreductase activity. The soluble form (AIFsol) found in the nucleus induces 'parthanatos' i.e. caspase-independent fragmentation of chromosomal DNA. Interacts with EIF3G,and thereby inhibits the EIF3 machinery and protein synthesis, and activates casapse-7 to amplify apoptosis. Plays a critical role in caspase- independent, pyknotic cell death in hydrogen peroxide-exposed cells. Binds to DNA in a sequence-independent manner.|
|Cellular Location||Mitochondrion intermembrane space. Mitochondrion inner membrane. Cytoplasm. Nucleus. Cytoplasm, perinuclear region. Note=Proteolytic cleavage during or just after translocation into the mitochondrial intermembrane space (IMS) results in the formation of an inner-membrane-anchored mature form (AIFmit). During apoptosis, further proteolytic processing leads to a mature form, which is confined to the mitochondrial IMS in a soluble form (AIFsol). AIFsol is released to the cytoplasm in response to specific death signals, and translocated to the nucleus, where it induces nuclear apoptosis. Colocalizes with EIF3G in the nucleus and perinuclear region Isoform 5: Cytoplasm|
|Tissue Location||Detected in muscle and skin fibroblasts (at protein level). Isoform 5 is frequently down-regulated in human cancers.|
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Provided below are standard protocols that you may find useful for product applications.
AIF (apoptosis inducing factor) was recently identified that causes chromatin condensation and DNA fragmentation. AIF translocates to the nucleus when apoptosis is induced and induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9. AIF induces chromatin condensation and DNA fragmentation, which are the hallmarks of apoptosis. AIF is highly conserved between human and mouse and widely expressed.
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