|Application ||WB, FC|
|Calculated MW||177439 Da|
|Application & Usage||Western blot analysis (1:100~500), ELISA ( ~1:1,000), Flow cytometry (1:10~50).|
|Formulation||100 µg (0.25 mg/ml) affinity purified rabbit anti-nestin polyclonal antibody in PBS with 0.09% (W/V) sodium azide.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||Nestin Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Required for brain and eye development. Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells (By similarity).|
|Tissue Location||CNS stem cells.|
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Provided below are standard protocols that you may find useful for product applications.
Nestin may play a role in the trafficking and distribution of IF proteins and potentially other cellular factors to da µghter cells during progenitor cell division (By similarity).
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