|Application ||WB, FC|
|Calculated MW||177439 Da|
|Application & Usage||Western blot analysis (1:100~500), ELISA ( ~1:1,000), Flow cytometry (1:10~50).|
|Formulation||100 µg (0.25 mg/ml) affinity purified rabbit anti-nestin polyclonal antibody in PBS with 0.09% (W/V) sodium azide.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||Nestin Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Required for brain and eye development. Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells (By similarity).|
|Tissue Location||CNS stem cells.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
email@example.com, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
Nestin may play a role in the trafficking and distribution of IF proteins and potentially other cellular factors to da µghter cells during progenitor cell division (By similarity).
If you have used an Abgent product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at firstname.lastname@example.org.