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SIRT3 Antibody
Rabbit Polyclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB |
|---|---|
| Primary Accession | Q9NTG7 |
| Reactivity | Human |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 43573 Da |
| Gene ID | 23410 |
|---|---|
| Positive Control | Jurkat cell lysate |
| Application & Usage | Western Blot analysis (0.5-4 µg/ml). However, the optimal concentrations should be determined individually. Blocking peptide is available separately. |
| Other Names | NAD-dependent deacetylase sirtuin-3, mitochondrial, SIR2-like protein 3 |
| Target/Specificity | Sirtuin-3 |
| Antibody Form | Liquid |
| Appearance | Colorless liquid |
| Formulation | 100 µg (0.5 mg/ml) affinity purified rabbit anti- SIRT3 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 5 mM EDTA and 0.01% thimerosal. |
| Handling | The antibody solution should be gently mixed before use. |
| Reconstitution & Storage | -20 °C |
| Background Descriptions | |
| Precautions | SIRT3 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | SIRT3 |
|---|---|
| Synonyms | SIR2L3 |
| Function | NAD-dependent protein deacetylase (PubMed:12186850, PubMed:12374852, PubMed:16788062, PubMed:18680753, PubMed:18794531, PubMed:23283301, PubMed:24121500, PubMed:24252090, PubMed:19535340). Activates or deactivates mitochondrial target proteins by deacetylating key lysine residues (PubMed:12186850, PubMed:12374852, PubMed:16788062, PubMed:18680753, PubMed:18794531, PubMed:23283301, PubMed:24121500, PubMed:24252090). Known targets include ACSS1, IDH, GDH, SOD2, PDHA1, LCAD, SDHA and the ATP synthase subunit ATP5PO (PubMed:16788062, PubMed:18680753, PubMed:24121500, PubMed:24252090, PubMed:19535340). Contributes to the regulation of the cellular energy metabolism (PubMed:24252090). Important for regulating tissue-specific ATP levels (PubMed:18794531). In response to metabolic stress, deacetylates transcription factor FOXO3 and recruits FOXO3 and mitochondrial RNA polymerase POLRMT to mtDNA to promote mtDNA transcription (PubMed:23283301). Acts as a regulator of ceramide metabolism by mediating deacetylation of ceramide synthases CERS1, CERS2 and CERS6, thereby increasing their activity and promoting mitochondrial ceramide accumulation (By similarity). Regulates hepatic lipogenesis. Uses NAD(+) substrate imported by SLC25A47, triggering downstream activation of PRKAA1/AMPK-alpha signaling cascade that ultimately downregulates sterol regulatory element-binding protein (SREBP) transcriptional activities and ATP-consuming lipogenesis to restore cellular energy balance. |
| Cellular Location | Mitochondrion matrix |
| Tissue Location | Widely expressed. |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
Silent information regulator (Sir2)-like family deacetylases (also known as sirtuins) are highly conserved proteins and have important roles in the regulation of metabolism, inflammation, cellular survival growth and differentiation. SIRT3 is a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Yeast sirtuin proteins are found to regulate epigenetic gene silencing. Sirtuins are NAD-dependent protein ADP-ribosyl transferase which catalyzes the transfer of ADP-ribosyl groups onto target proteins.
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