MBD2a Antibody
Rabbit Polyclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB |
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Primary Accession | B0BNM2 |
Other Accession | B0BNM2 |
Reactivity | Human, Mouse, Rat |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Positive Control | Jurkat cell lysate |
---|---|
Application & Usage | The antibody can be used in Western Blot analysis (0.5-4 µg/ml). However, the optimal concentrations should be determined individually. Blocking peptide is available separately. |
Other Names | Methyl-CpG-binding domain protein 2 |
Target/Specificity | MBD2a |
Antibody Form | Liquid |
Appearance | Colorless liquid |
Formulation | 100 µg (0.5 mg/ml) affinity purified rabbit anti- MBD2a polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 5 mM EDTA and 0.01% thimerosal. |
Handling | The antibody solution should be gently mixed before use. |
Reconstitution & Storage | -20 °C |
Background Descriptions | |
Precautions | MBD2a Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
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Provided below are standard protocols that you may find useful for product applications.
Background
The MBD family of proteins includes MeCP2, MBD1, MBD2, MBD3 and MBD4. MBD2 is highly similar to MBD3 but only MBD2 targets the MeCP1 complex to methylate DNA. MBD2 has two isoforms, MBD2a and MBD2b. MBD2a is a 414 amino acids protein, whereas MBD2b lacks the 152 amino acids N-terminal extension. MBD2a interacts with RNA helicase A to enhance CREB-dependent gene exptression.
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