|Reactivity||Human, Mouse, Rat|
|Positive Control||Rat kidney tissue lysate|
|Application & Usage||Western Blot analysis (0.5-4 µg/ml). However, the optimal concentrations should be determined individually. Blocking peptide is available separately.|
|Other Names||Enhancer of zeste homolog 2 (ENX-1), isoform CRA_b|
|Formulation||100 µg (0.5 mg/ml) affinity purified rabbit anti- EZH2 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 5 mM EDTA and 0.01% thimerosal.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||EZH2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
Enhancer of zeste homolog 2 (Ezh2) is a member of the Polycomb group (PcG). The PcG proteins are involved in maintaining the silenced state of several developmentally regulated genes and contribute to the maintenance of cell identity, cell cycle regulation, and oncogenesis. Enhancer of zeste homolog 2 (Ezh2) contains four conserved regions including domain I, domain II, and a cysteine-rich amino acid stretch that precedes the carboxy-terminal SET domain. Ezh2 also plays a role as primary effector and as a mediator of tumorigenesis.
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