|Application ||WB, IP|
|Calculated MW||40451 Da|
|Application & Usage||Western blot (at 0.5-4 µg/ml) and immunoprecipitation. However, the optimal conditions should be determined individually.|
|Other Names||guanine nucleotide binding protein G, GNAI2B, guanine nucleotide binding protein|
|Formulation||100 µg (0.5 mg/ml) affinity-purified rabbit anti-Gαi2 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||Gai2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. The G(i) proteins are involved in hormonal regulation of adenylate cyclase: they inhibit the cyclase in response to beta-adrenergic stimuli. May play a role in cell division.|
|Cellular Location||Cytoplasm. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cell membrane. Note=Localizes in the centrosomes of interphase and mitotic cells. Detected at the cleavage furrow and/or the midbody|
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Provided below are standard protocols that you may find useful for product applications.
Gαi2 is a 40-41 kDa alpha-subunit of heterotrimeric protein that shares sequence homology with Gαi3 and Gαi1. All these G proteins can be ADP-ribosylated at the carboxy terminal Cys of the CAAX sequence, interfering with receptor coupling. Gαi2 is expressed widely in tissues and cell lines. Activation of PLC-β by Gαi-coupled receptors is the result of release of Gβg subunits from Gαi. Gαi2 regulates potassium channel activation. Mutation at Arg179 has been identified in tumors. This and the Q205L mutant inhibit adenylyl cyclase.
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