|Calculated MW||35071 Da|
|Application & Usage||Western blot (0.5-4 µg/ml). However, the optimal conditions should be determined individually. Cross-reactivity to IkB-alpha of other species has not been determined.|
|Other Names||NFKBIA, IKBA, MAD3, MAD-3, IkappaBalpha, I-kappa-B-alpha, # NFKBI, IkB-alpha, NF-kappa-B inhibitor alpha; I-kappa-B-alpha; IkappaBalpha; IkB-alpha|
|Formulation||100 µg (0.2 mg/ml) affinity-purified rabbit anti-IkB-alpha polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 50% glycerol, 1% BSA, and 0.02% thimerosal.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||IkBalpha Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription.|
|Cellular Location||Cytoplasm. Nucleus. Note=Shuttles between the nucleus and the cytoplasm by a nuclear localization signal (NLS) and a CRM1-dependent nuclear export.|
|Tissue Location||Highly expressed in lymph node, thymus followed by liver, brain, muscle, kidney, gastrointestinal and reproductive tract|
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Provided below are standard protocols that you may find useful for product applications.
IkB-alpha protein is a NF-kB inhibitory protein. It binds NF-kB and retains the complex in the cytoplasm, thereby preventing it from entering into nucleus and functioning as a transcription factor. Upon receiving a variety of signals, IkB-alpha undergoes phosphorylation at serine residues by an ubiquitin-dependent protein kinases and finally degraded by the proteasome. Free NF-kB then binds DNA and affects gene expression.
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