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IkBalpha Antibody
Rabbit Polyclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB |
|---|---|
| Primary Accession | Q9Z1E3 |
| Reactivity | Mouse |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 35071 Da |
| Gene ID | 18035 |
|---|---|
| Application & Usage | Western blot (0.5-4 µg/ml). However, the optimal conditions should be determined individually. Cross-reactivity to IkB-alpha of other species has not been determined. |
| Other Names | NFKBIA, IKBA, MAD3, MAD-3, IkappaBalpha, I-kappa-B-alpha, # NFKBI, IkB-alpha, NF-kappa-B inhibitor alpha; I-kappa-B-alpha; IkappaBalpha; IkB-alpha |
| Target/Specificity | IkBalpha |
| Antibody Form | Liquid |
| Appearance | Colorless liquid |
| Formulation | 100 µg (0.2 mg/ml) affinity-purified rabbit anti-IkB-alpha polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 50% glycerol, 1% BSA, and 0.02% thimerosal. |
| Handling | The antibody solution should be gently mixed before use. |
| Reconstitution & Storage | -20 °C |
| Background Descriptions | |
| Precautions | IkBalpha Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | Nfkbia |
|---|---|
| Synonyms | Ikba |
| Function | Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL (RELA/p65 and NFKB1/p50) dimers in the cytoplasm by masking their nuclear localization signals (PubMed:10097128, PubMed:9990853). On cellular stimulation by immune and pro-inflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription (PubMed:7878466, PubMed:10097128, PubMed:9990853). |
| Cellular Location | Cytoplasm {ECO:0000250|UniProtKB:P25963}. Nucleus {ECO:0000250|UniProtKB:P25963}. Note=Shuttles between the nucleus and the cytoplasm by a nuclear localization signal (NLS) and a CRM1- dependent nuclear export. {ECO:0000250|UniProtKB:P25963} |
| Tissue Location | Highly expressed in lymph node, thymus followed by liver, brain, muscle, kidney, gastrointestinal and reproductive tract |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
IkB-alpha protein is a NF-kB inhibitory protein. It binds NF-kB and retains the complex in the cytoplasm, thereby preventing it from entering into nucleus and functioning as a transcription factor. Upon receiving a variety of signals, IkB-alpha undergoes phosphorylation at serine residues by an ubiquitin-dependent protein kinases and finally degraded by the proteasome. Free NF-kB then binds DNA and affects gene expression.
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